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"内細胞と細胞骨格の動態の拡張解像度構造化照明イメージング"に関するコメントへの応答

Dong Li¹, Eric Betzig²

¹National Laboratory of Biological Macromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, P.R. China. Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147. lidong@ibp.ac.cn betzige@janelia.hhmi.org.

Science (New York, N.Y.)

|April 30, 2016

まとめ

この研究は,画像アーティファクト,解像度基準,および研究中の生細胞の性能に関する批判に対応しています. Sahl et al.が提起した各項目の詳細な回答を掲載しています.

科学分野:

顕微鏡検査
細胞生物学
画像分析

背景:

Sahl et al が懸念を表明した. 出版物の特定の側面についてです.
これらの批判は,画像アーティファクト,解像度基準,および生細胞の性能評価に焦点を当てました.

研究の目的:

Sahl et al が提唱した批判を体系的に取り上げ,反論する.
方法論的な詳細を明らかにし,我々の最初の研究の結果を検証する.

主な方法:

画像データを再分析し,潜在的なアーティファクトを特定し,説明する.
画像品質を評価するために確立された解像度基準の適用
生体細胞サンプルでの比較性能試験

主要な成果:

観察された現象は,画像アーティファクトによるものではありません.
解決基準が適切に満たされていることを確認する.
生細胞での比較性能の検証

結論:

Sahl et al による批判私たちの再評価に基づいています.
当初の発見と方法論は堅実で科学的に健全です
この回答は重要な点を明らかにし,私たちの研究の妥当性を再確認します.

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