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RNA Structure01:19

RNA Structure

8.1K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
8.1K
RNA Structure01:23

RNA Structure

80.2K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
80.2K
RNA Structure01:23

RNA Structure

29.7K
29.7K
RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.4K
Ribosome Profiling02:24

Ribosome Profiling

4.3K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.3K
Nucleic Acid Structure01:25

Nucleic Acid Structure

10.0K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
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関連する実験動画

Updated: Mar 21, 2026

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
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Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Published on: May 24, 2017

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生体 細胞 の RNA デュプレックス マップ は,より 高い 順序 の トランスクリプトーム の 構造 を 明らかに し て い ます

Zhipeng Lu1, Qiangfeng Cliff Zhang2, Byron Lee1

  • 1Center for Personal Dynamic Regulomes, Stanford University, Stanford, CA 94305, USA.

Cell
|May 17, 2016
PubMed
まとめ

私たちはPARISを開発しました 生きた細胞のRNA構造をマッピングする 新しい方法です この技術は,広範囲にわたる複雑なRNA構造と相互作用を明らかにし,RNA構造体とインタラクトームに関する新しい洞察を提供します.

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Estimation of Telomeric Repeat-containing RNA from DNA/RNA Hybrid Complexes
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Estimation of Telomeric Repeat-containing RNA from DNA/RNA Hybrid Complexes

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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

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関連する実験動画

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Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
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Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
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科学分野:

  • 分子生物学
  • 遺伝学
  • 生物化学

背景:

  • RNAの塩基配列は 生物学的機能の多様性にとって 極めて重要です
  • RNAの構造と相互作用を理解することは 細胞のプロセスを解読するのに不可欠です

研究 の 目的:

  • 高解像度で生きている細胞のRNA複合体のグローバルマッピングの方法を開発する.
  • トランスクリプトーム全体でのRNA構造と相互作用の様子を調査する.

主な方法:

  • PARIS (RNAの相互作用と構造のためのpsoralenクロスリンク) を開発し,可逆性psoralenクロスリンクを使用した方法.
  • PARISは,ヒトとマウスの細胞型で,塩基対に近い解像度でRNA複合体をマッピングするために適用された.
  • PARISのデータを利用してRNA構造の遺伝分析を導いた.

主要な成果:

  • トランスクリプトーム内の頻繁な長距離RNA構造と高級アーキテクチャを特定した.
  • 単一の分子レベルで 広範囲に広がる代替RNAの形状を明らかにした.
  • X染色体不活性化に不可欠なXIST lncRNAの複合複合を含む,保存された長距離および代替RNA構造を発見した.

結論:

  • PARISは,RNA構造体とインタラクトムのグローバルマッピングのための汎用的なツールを提供します.
  • この研究では,以前は特徴づけられなかった広範囲で複雑なRNA構造と相互作用が明らかにされました.
  • PARISベースの構造的洞察は,XIST RNAの表遺伝子静止における役割によって例示されるRNAの機能,進化,および調節を理解するのに役立ちます.