核局所化された周期性核酸ゲートチャネルは,共生カルシウム振動を媒介する

Myriam Charpentier¹, Jongho Sun², Teresa Vaz Martins³

¹Cell and Developmental Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK. myriam.charpentier@jic.ac.uk giles.oldroyd@jic.ac.uk.

Science (New York, N.Y.)

|May 28, 2016

まとめ

植物の根は核カルシウム (Ca2+) の振動を利用して有益な微生物と交流します 3つのサイクルヌクレオチドゲートチャネルがこれらの重要な共生シグナル伝達経路の主要要素として特定されました.

科学分野:

植物分子生物学
植物と微生物の相互作用
セルラー信号

背景:

核に関連したCa2+) 振動は,リゾビアや樹状菌根菌などの有益な微生物との共生に不可欠です.
カリウムに浸透するチャネルが関与していることは知られていますが,これらの振動を媒介する特定のカルシウムチャネルは特定されていません.

研究の目的:

植物の共生反応における核のCa2+) 振動に起因するカルシウムチャネルを特定する.
植物と有益な微生物間のコミュニケーションを媒介するこれらのチャネルの役割を明らかにする.

主な方法:

モデル豆類のメディカゴ・トランカチュラ (Medicago truncatula) の3つのサイクルヌクレオチドゲートチャネル (CNGC) を調査した.
CNGCの核封筒の位置と機能を決定するために利用された技術.
CNGCとシンビオティックシグナル伝達に関与する既知のカリウム透過チャネルとの相互作用を調べた.

主要な成果:

核のCa2+振動に不可欠な3つのCNGCをMedicago truncatulaで特定した.
これらのCNGCは核膜に位置し,Ca2+に浸透性があることが示されています.
CNGCは,シンバイオシス中に核のCa2+放出を調節する,カリウム浸透性チャネルを持つ複合体を形成することを示した.

結論:

循環性ヌクレオチドゲートチャネルは植物-微生物共生における核カルシウムシグナル伝達機構の重要な構成要素です
これらの発見は共生シグナル伝達に関与する新しいカルシウムチャネルを明らかにし,動物細胞で見つかったものと類似しています.
特定されたチャネルは,様々な環境と開発のシグナルに対する核Ca2+) の反応を調節する上でより広範な役割を果たす可能性が高い.

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