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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR01:59

CRISPR

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18.8K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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RNA Interference01:23

RNA Interference

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Updated: Mar 20, 2026

CRISPR Guide RNA Cloning for Mammalian Systems
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CRISPR Guide RNA Cloning for Mammalian Systems

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C2c2は,1つのコンポーネントのプログラム可能なRNA誘導型RNAターゲティングCRISPRエフェクターである.

Omar O Abudayyeh1, Jonathan S Gootenberg2, Silvana Konermann3

  • 1Department of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA. McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA. Departments of Brain and Cognitive Science and Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Science (New York, N.Y.)
|June 4, 2016
PubMed
まとめ

研究者は,C2c2 CRISPR-Casエフェクター,新しいRNA誘導リボヌクレアスを特徴付けました. Leptotrichia shahiiのこのシステムは 特定のRNA分子を標的にし 分割し 新しいRNA標的にするツールを提供します

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Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx
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A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
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A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

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関連する実験動画

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CRISPR Guide RNA Cloning for Mammalian Systems
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Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx
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A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
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科学分野:

  • 微生物学
  • 分子生物学
  • 生物化学

背景:

  • クラスタリングされた定期的な間隔の短いパリンドロミックリピート (CRISPR) - CRISPR関連遺伝子 (Cas) システムは,微生物の適応免疫を提供します.
  • CRISPR-Casシステムは DNAまたはRNA-DNAの干渉によって 異質の遺伝子要素に対して機能します
  • クラス2のCRISPR-Casエフェクターは,これらの微生物の防御メカニズムの別々のカテゴリーを表しています.

研究 の 目的:

  • クラス2型CRISPR-CasエフェクターC2c2を特徴付ける
  • C2c2のRNA誘導リボヌクレアース活性を示すために.
  • 新しいRNAターゲティングツールとしてのC2c2の可能性を調査する.

主な方法:

  • Leptotrichia shahiiからのC2c2の生化学分析
  • RNAの分裂活性を評価するための in vitro 測定.
  • 保存されたHigher Eukaryotesおよび Prokaryotes Nucleotide-binding (HEPN) ドメインの変異.

主要な成果:

  • C2c2は,単一鎖RNAを標的としたRNA誘導リボヌクレアース活性を示す.
  • C2c2はバクテリアのRNAファグに対する干渉を媒介する.
  • 特定のmRNAのノックダウンは,C2c2のプログラムによってバクテリアで達成された.
  • 触媒活動は,HEPN領域内の保存された残留物に依存しています.

結論:

  • C2c2はRNA誘導リボヌクレアースであり,RNAターゲティングに潜在的応用がある.
  • C2c2を理解することで,CRISPR-Casシステムの多様性と機能に関する知識が広がります.
  • C2c2はRNAの操作のための新しいツールに設計することができます.