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クロマチンの改造には,NUDIX5によるADP-リボース由来核ATP合成が必要です

Roni H G Wright¹, Antonios Lioutas¹, Francois Le Dily¹

¹Centre de Regulació Genòmica (CRG), Barcelona Institute for Science and Technology, Barcelona E-09003, Spain. Universitat Pompeu Fabra, Barcelona E-08003, Spain.

Science (New York, N.Y.)

|June 4, 2016

まとめ

核のATP生成は DNA修復や遺伝子調節のような細胞の重要なプロセスを促進しますこの研究は,NUDIX5によるポリアドプリボース水解が,ホルモン誘発の乳がん細胞反応に不可欠な核ATPを生成することを明らかにしています.

科学分野:

分子生物学
細胞の代謝
エピジェネティクス

背景:

核プロセス (DNA複製,修復,遺伝子調節) には ATP が必要です.
クロマチンの改造はエネルギー密集的で酵素によって触媒されます
急速な刺激への反応のための核ATPの源はほとんど不明である.

研究の目的:

刺激に反応する核ATP生成のメカニズムを調査する.
乳がん細胞がプロゲスティン誘発の遺伝子調節で ATPの需要を満たす方法を理解する.

主な方法:

核におけるポリアドプリボース水解の分析.
ATP合成におけるNUDIX5の役割を調査した.
ホルモンの誘発によるクロマチンの改造と遺伝子発現の変化を研究した.

主要な成果:

ATPは,ポリ ((ADP-リボース) からADP-リボースへの水解によって核で生成される.
酵素NUDIX5はADP-リボースとピロホスファートを利用して核ATPを生成する.
この核ATPはホルモン誘発の染色体改造,遺伝子調節,増殖に不可欠です.

結論:

核ATP生成のための新しい経路が,ポリ (ADP-リボース) 水解によって特定されました.
NUDIX5は細胞プロセスにおける核ATP生成の鍵となる酵素です.
核ATP合成は,乳がん細胞がプロゲスティンに反応するのに不可欠です.

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