関連する実験動画

Updated: Mar 19, 2026

Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks
11:04

Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks

Published on: December 24, 2016

10.3K

Vivoでは,設計されたソルターゼ変種を用いた様々なアミンによるサイト固有のタンパク質タグ付けを行う

Jeff E Glasgow1, Marc L Salit1, Jennifer R Cochran2

  • 1National Institute of Standards and Technology , Stanford, California 94305, United States.

Journal of the American Chemical Society
|June 10, 2016
PubMed
まとめ

この研究は,エンジニアリングされた酵素変種を用いた新しいタンパク質改変法を示しています. このアプローチは,活体細菌内でさえも,多様な化学タグを持つ機能化されたタンパク質の費用対効果の高い生産を可能にし,浄化の必要性を排除します.

さらに関連する動画

Cell-Type Specific Protein Purification and Identification from Complex Tissues Using a Mutant Methionine tRNA Synthetase Mouse Line07:39

Cell-Type Specific Protein Purification and Identification from Complex Tissues Using a Mutant Methionine tRNA Synthetase Mouse Line

Published on: April 13, 2022

3.5K
Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines11:32

Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines

Published on: November 13, 2021

11.7K

関連する概念動画

Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
8.7K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
7.3K