長い非コーディングRNA lincRNA-EPSは,炎症を抑制する転写ブレーキとして作用する
Maninjay K Atianand1, Wenqian Hu2, Ansuman T Satpathy3
1Program in Innate Immunity, University of Massachusetts Medical School, Worcester, MA 01605, USA.
Cell
|June 18, 2016
まとめ
長い非コーディングRNA (lncRNAs) は遺伝子発現を調節する. マクロファージの炎症性遺伝子発現を抑制する 新種のリンクRNA-EPSを発見しました 免疫反応の調節に不可欠です
科学分野:
- 免疫学
- 分子生物学
- 遺伝学
背景:
- 長いインター遺伝子非コーディングRNA (lincRNAs) は,遺伝子発現の新興レギュレータである.
- 免疫細胞の機能における lncRNA の役割は,大部分は未定義のままである.
- マクロファージは炎症反応に関与する 重要な免疫細胞です
研究 の 目的:
- 新しい免疫調節リンカーンRNAを特定し,特徴づけること.
- マクロファージ媒介の免疫反応における lincRNA- EPS の機能を明らかにする.
- lincRNA-EPSが遺伝子発現を制御する分子機構を調査する.
主な方法:
- lincRNA-EPS欠乏性のマクロファージのトランスクリプトーム解析
- マクロファージの機能増強と救出実験
- リンクRNA- EPS欠乏症のマウスを用いたインビボ試験では,エンドトキシンを投与した.
- リンクRNAの局所化と相互作用を決定するためのクロマチンの免疫降水とRNA結合測定法.
主要な成果:
- lincRNA- EPSはマクロファージで正確に調節され,免疫応答遺伝子 (IRG) の発現を抑制する.
- lincRNA- EPS欠乏したマウスは,エンドトキシンの刺激で炎症と死亡率を高めます.
- lincRNA- EPSは,IRGの規制領域に局所化し,核細胞の位置づけに影響を与え,転写を抑制する.
- lincRNA- EPSは,特殊なモチーフを通して異質な核リボ核タンパク質L (hnRNP L) と相互作用し,その抑制機能を媒介する.
結論:
- lincRNA- EPSはマクロファージにおける炎症性遺伝子発現の重要な抑制剤として作用する.
- この研究は,先天的な免疫の調節における lincRNAs の重要な役割を強調しています.
- lincRNA- EPSは炎症性疾患の潜在的治療標的である.
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