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Updated: Mar 18, 2026

PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
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PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase

Published on: September 26, 2020

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トリプトファン合成のエンジニアリングされたサブユニットを使用してβ-分岐トリプトファンアナログの合成

Michael Herger1, Paul van Roye1, David K Romney1

  • 1Division of Chemistry and Chemical Engineering 210-41, California Institute of Technology , 1200 East California Boulevard, Pasadena, California 91125, United States.

Journal of the American Chemical Society
|June 30, 2016
PubMed
まとめ

エンジニアリングされたトリプトファン合成は,2S,3S) -β-メチルトリプトファン (β-MeTrp) の1段階合成をl-スレオニンを使用して可能にします. 誘導進化は酵素活性を大幅に高め,貴重なβ-MeTrp誘導体へのより簡単な経路を提供しました.

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