Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Proteomics01:33

Proteomics

10.1K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
10.1K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

8.8K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.8K
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

7.4K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
7.4K
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

2.8K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.8K
Protein Complex Assembly02:41

Protein Complex Assembly

2.7K
2.7K
Protein Complex Assembly02:41

Protein Complex Assembly

17.0K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
17.0K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Quantifying species-specific binding affinities of transthyretin aggregation inhibitors.

Biophysical reports·2026
Same author

Tracking the redox reaction of the iron enzyme ribonucleotide reductase using continuous SerialED and SFX.

Structure (London, England : 1993)·2026
Same author

Structural basis of the promiscuity of the unusual Fe(II) and 2-oxoglutarate dependent human aspartate/asparagine-β-hydroxylase.

Nature communications·2026
Same author

Linker Length and Composition within Disordered Binding Motifs Modulates the Avidity and Reversibility of a Multivalent Protein Interaction Switch.

Journal of molecular biology·2025
Same author

Mechanism of inhibition of acid-mediated transthyretin aggregation by designed peptides.

The Journal of biological chemistry·2025
Same author

Client-scaffold interactions suppress aggregation of a client protein in model condensates.

Proceedings of the National Academy of Sciences of the United States of America·2025

関連する実験動画

Updated: Mar 18, 2026

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
09:00

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

Published on: April 18, 2025

1.5K

暗黒 の プロテオム の 中 で 道 を 見いだす

Asmit Bhowmick, David H Brookes, Shane R Yost

  • 1Department of Integrative Structural and Computational Biology, Scripps Research Institute , La Jolla, California 92037, United States.

Journal of the American Chemical Society
|July 9, 2016
PubMed
まとめ

固有の無秩序なタンパク質 (IDP) は 伝統的な構造生物学の課題です 新しい統合された実験的および計算的アプローチは,それらの機能を明らかにするために必要です.

さらに関連する動画

A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease
09:52

A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease

Published on: January 10, 2025

1.5K
Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
14:51

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

6.3K

関連する実験動画

Last Updated: Mar 18, 2026

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
09:00

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

Published on: April 18, 2025

1.5K
A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease
09:52

A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease

Published on: January 10, 2025

1.5K
Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
14:51

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

6.3K

科学分野:

  • 構造生物学
  • コンピュータ生物学
  • バイオ物理学

背景:

  • X線結晶学のような 伝統的な方法は 折りたたまれたタンパク質に優れています
  • 人間のプロテオームの大部分は,本質的に乱れたタンパク質と領域 (IDP/IDR) で構成されています.
  • IDP/IDRには安定した構造がないため,従来の技術で研究することが困難であり",ダーク・プロテオーム"に寄与する.

研究 の 目的:

  • 本質的に無秩序なタンパク質 (IDP) の構造アンサンブルを特徴づけるための課題に取り組む.
  • "ダーク・プロテオーム"を研究するための統合された実験と計算戦略の必要性を探求する.

主な方法:

  • 多様な解決策に基づく実験を統合する.
  • 最先端の分子シミュレーションを使って
  • ベイジアン確率モデルと 高通量計算を適用する.
  • "計算上のビームライン"の概念を開発する.

主要な成果:

  • 従来の方法は,IDPのダイナミックな形状のサブ状態を捉えることができない.
  • IDPの構造を決定するには,様々なテクニックを組み合わせた多面的なアプローチが必要です.
  • 提案された"計算ビームライン"は実験データと計算データを統合しています.

結論:

  • "ダーク・プロテオーム"を理解するには 新しい統合的アプローチが不可欠です
  • "計算ビームライン"は,IDP構造アンサンブル内の機能的特徴を特定するための経路を提供します.
  • この展望は,本質的に無秩序なタンパク質の研究の将来の方向性を強調しています.