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関連する概念動画

The Proteasome01:13

The Proteasome

1.9K
Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
1.9K
The Proteasome02:18

The Proteasome

10.4K
Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
10.4K
The Proteasome02:18

The Proteasome

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5.0K
Regulated Protein Degradation02:58

Regulated Protein Degradation

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It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
9.1K
Regulated Protein Degradation02:58

Regulated Protein Degradation

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3.3K
The Proteasome Structure01:17

The Proteasome Structure

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The ubiquitin-proteasome pathway is a well-known mechanism utilized by eukaryotic cells to remove cytoplasmic proteins that are misfolded, damaged, or no longer needed. In this pathway, the protein that needs to be eliminated undergoes a process called ubiquitination, where a chain of ubiquitin molecules is attached to the 48th lysine residue of the target protein. This ubiquitin modification helps the proteasome distinguish between a target protein and a healthy protein.
The proteasome is an...
2.1K

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関連する実験動画

Updated: Mar 17, 2026

Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans
09:18

Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans

Published on: September 7, 2021

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進化的に保存された経路がプロテアソームのホメオスタシスを制御する

Adrien Rousseau, Anne Bertolotti

    Nature
    |July 28, 2016
    PubMed
    まとめ

    保存されたシグナル伝達経路は プロテアソムのレベルを調節する. TORC1を阻害すると,Mpk1が活性化され,ストレス下での細胞生存のためのプロテアソーム成分が増加します. この経路は哺乳類に保存されています

    科学分野:

    • 細胞生物学
    • 分子生物学
    • 生物化学

    背景:

    • プロテアゾームはタンパク質の分解に不可欠ですが,その豊富さを維持するメカニズム (プロテアゾームホメオスタシス) は完全に理解されていません.
    • 細胞のプロテアソームレベルは,タンパク質のターンオーバーに対する細胞の要求を満たすために厳格に規制されなければならない.

    研究 の 目的:

    • プロテアソームのホメオスタシスを制御するシグナル伝達経路を解明する.
    • プロテアソームサブユニットとアセンブリチャペロン生産を制御する重要な調節体を特定する.

    主な方法:

    • TORC1とMpk1の役割を研究するために,酵母遺伝学と分子生物学技術が採用されました.
    • 哺乳類の細胞培養と分子分析を用いて,進化の保存を評価した.

    主要な成果:

    • 酵母におけるTORC1 (ラパミシン複合体1の標的) の阻害は,19S調節性粒子の集合チャペロン (RAC) とプロテアソームサブユニットの発現を誘導した.
    • TORC1のダウンストリームでは,Mpk1 (ミトゲン活性化タンパク質キナーゼ) 経路が活性化され,RACとプロテアソームサブユニットを増加させ,それによってプロテアソームの分解とストレス下での細胞活性を維持した.
    • mTOR (哺乳類のTOR) とERK5 (細胞外信号調節キナーゼ5) を含む経路は,哺乳類で保存され,RACレベルとプロテアソームの豊富さを制御することが判明しました.

    さらに関連する動画

    Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae
    09:05

    Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae

    Published on: April 18, 2016

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    Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism
    12:38

    Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism

    Published on: December 18, 2013

    6.6K

    関連する実験動画

    Last Updated: Mar 17, 2026

    Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans
    09:18

    Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans

    Published on: September 7, 2021

    3.4K
    Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae
    09:05

    Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae

    Published on: April 18, 2016

    30.2K
    Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism
    12:38

    Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism

    Published on: December 18, 2013

    6.6K

    結論:

    • TORC1/mTORとMpk1/ERK5を含む保存されたシグナル伝達経路は,細胞のニーズとストレスに反応して迅速にプロテアソームを調整します.
    • この適応反応は,プロテアソームの分解と細胞活性を維持するために不可欠です.
    • この経路を標的とした治療は,タンパク質分解の障害を特徴とする疾患の潜在的治療戦略です.