個々のE. coli細胞における複製と分裂サイクルの同期
Mats Wallden1, David Fange1, Ebba Gregorsson Lundius1
1Department of Cell and Molecular Biology, Science for Life Laboratory, Uppsala University, Husargatan 3, 75124 Uppsala, Sweden.
Cell
|July 30, 2016
まとめ
E. coli の成長における細胞の変動は,染色体複製の開始のための固定された体積トリガーによって説明されます. このメカニズムは"アドラー"の行動を誘導し 細胞は世代ごとに一貫したサイズを増やします
科学分野:
- 微生物学
- 細胞生物学
- システム生物学
背景:
- イソジェニック・エシェリキア・コライ (E. coli) 集団は,恒常的な環境条件下でも,細胞間での成長率,分裂サイズ,生成時間の有意な変動を示します.
- 注目すべき点は,
- ヘビ
- 細胞の成長は 生まれた時のサイズとは無関係です
研究 の 目的:
- E. coli の根本的なメカニズムを調査する
- ヘビ
- 行動についてです
- 染色体複製と細胞分裂サイクルとの関係をマッピングする.
主な方法:
- 個々のE. coli細胞をモニターするために光顕微鏡を用いた.
- 染色体複製の開始と細胞分裂をリアルタイムで追跡した.
主要な成果:
- 染色体複製の開始は,出生量または成長率に関係なく,染色体ごとに固定された細胞量で引き起こされます.
- 各複製開始イベントは,成長率に依存する時間の経過後に,その後の細胞分裂イベントと関連しています.
- 細胞間での分裂のタイミングと細胞サイズの変化は,主に成長率の変動によって引き起こされます.
結論:
- この研究は,観察された大腸菌のメカニズム的説明を提供する.
- ヘビ
- 行動についてです
- このモデルは 急速成長する細胞が 増殖行動を示す理由を説明し 成長速度が遅い場合の 偏差を予測します
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