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関連する概念動画

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution00:52

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

1.4K
At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
1.4K
NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

1.1K
When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
1.1K
NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

1.9K
A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
1.9K
Chemical Shift: Internal References and Solvent Effects01:17

Chemical Shift: Internal References and Solvent Effects

1.5K
In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...
1.5K
¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR01:15

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

1.8K
The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.
1.8K
Applications Of NMR In Biology01:25

Applications Of NMR In Biology

4.7K
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
4.7K

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Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
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(129) Xe NMR リラクゼーションベースのマクロモレキュラーセンシング

Muller D Gomes1,2, Phuong Dao1,2, Keunhong Jeong1,2

  • 1Materials Sciences Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.

Journal of the American Chemical Society
|July 30, 2016
PubMed
まとめ

この研究では新しいクセノン-129 NMR リラクゼーションセンサが導入されます. この方法は,標的結合時にクセノンのリラックス率の変化を測定することによって,大きな分子の感度検出を可能にします.

さらに関連する動画

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the &#181;s-ms Timescale
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15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

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Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
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Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

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関連する実験動画

Last Updated: Mar 17, 2026

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
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15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the &#181;s-ms Timescale
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Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
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科学分野:

  • 核磁共振 (NMR) スペクトロスコーピー
  • 化学センサー
  • バイオ分子相互作用分析

背景:

  • 核磁共振 (NMR) のリラクゼーションは分子運動に敏感です.
  • クリプトファンのケージはクセノン (Xe) を封じ込み,ターゲット認識のために機能化することができます.
  • センシングアプリケーションには,しばしば感度を増やすための増幅戦略が必要です.

研究 の 目的:

  • 大分子標的を検出するためのクセノン-129 NMR リラクゼーションベースのセンシングアプローチを開発する.
  • 大量溶解したクセノンの増幅信号を利用して感知する.
  • 標的の結合,センサの転落,そしてクセノンの放松率との関係を調査する.

主な方法:

  • 標的の相互作用要素と金属合剤で機能する暗号ファンのベースのセンサーが設計されました.
  • センサーはアビディンに結合するバイオチニル標的を検出するために使用されました.
  • 大量 (129) Xe NMR リラクゼーション率 (T2) の変化は,標的結合時に測定された.

主要な成果:

  • センサへの標的の結合は,カプセル化されたクセノンの回転相関時間を大幅に変化させた.
  • この変化により,大量クセノン放緩率の測定可能な増加が発生した.
  • バイオチン含有センサーを1.5μMでアビディンに結合すると,自由クセノンT2は4倍に減少し,感度が高いことが示された.

結論:

  • 開発された (129) Xe NMR リラクゼーションベースのセンシングアプローチは,大規模なマクロ分子標的を効果的に検出します.
  • 大量溶解したクセノンの増幅により,分析物の感度が高くなります.
  • この方法は,NMRを用いた 敏感な生物分子検出のための 新しい有望な方法を提供します.