関連する実験動画
Updated: Mar 16, 2026

04:28
Lung Tumor Cell Recruitment Assay
Published on: February 26, 2019
6.1K
まとめ
腫瘍細胞は内皮細胞のプログラム細胞死 (ネクロプトーシス) を誘発し,癌の転移を促進する. RIPK1またはDR6を介してこの経路を阻害することは,潜在的な抗転移療法を提供します.
科学分野:
- 腫瘍学
- 細胞生物学
- 分子医学
背景:
- 癌細胞の広がりである転移は 癌による死亡の主な原因です
- 腫瘍細胞の拡張は 転移の重要なステップで 内皮の障壁を通過します
- 腫瘍細胞と内皮細胞の相互作用を制御する分子機構は完全に理解されていません.
研究 の 目的:
- 腫瘍細胞が内皮壁を通過する分子のメカニズムを解明する.
- 腫瘍転移中の内皮細胞におけるプログラム細胞死 (ネクロプトーシス) の役割を調査する.
- 癌の転移を抑制する潜在的治療標的を特定する.
主な方法:
- 腫瘍細胞と内皮細胞の相互作用を in vitro と in vivo で研究した.
- 受容体と相互作用するセリン/スレオニンタンパク質キナーゼ1 (RIPK1) 阻害体ネクロスタチン-1と,内皮細胞におけるRIPK3とカスパース-8の遺伝子操作を用いた.
- アミロイド前駆体タンパク質 (APP) と死亡受容体6 (DR6) が腫瘍細胞の増殖を媒介する役割を調査した.
主要な成果:
- 腫瘍細胞は内皮細胞の死滅を引き起こし,外流と転移を促進します.
- RIPK1またはRIPK3の阻害は,腫瘍細胞誘発の内皮死,外流,転移を減少させた.
- カスパースの抑制またはカスパース-8の喪失は,これらの転移プロセスを促進しました.
- 腫瘍細胞で発現するAPPと内皮細胞で発現するDR6は,この死滅によって引き起こされるエクストラバゼーションの主要な媒介体として特定された.
結論:
- 腫瘍細胞誘発性内皮死は,がん細胞の拡張と転移を促進する新しいメカニズムです.
- DR6媒介の内皮細胞信号経路をターゲットにすることは,抗転移治療のための有望な戦略です.
さらに関連する動画
09:42Assessing Tumor Microenvironment of Metastasis Doorway-Mediated Vascular Permeability Associated with Cancer Cell Dissemination using Intravital Imaging and Fixed Tissue Analysis
Published on: June 26, 2019
9.3K
05:48Tissue Engineering of Tumor Stromal Microenvironment with Application to Cancer Cell Invasion
Published on: March 18, 2014
10.2K
関連する概念動画
Metastasis
6.8K
Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
6.8K
The Extrinsic Apoptotic Pathway
9.2K
The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
9.2K
Overview of Cell Death
10.9K
Cell death is an essential process where the body gets rid of old or damaged cells. Cell proliferation and death need to be balanced, as an imbalance between the two may lead to cancer or autoimmune diseases.
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...
10.9K
Necrosis
7.1K
Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”.
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...
7.1K
Cancer Cell Migration through Invadopodia
3.4K
Invadosome is a broad category of cell surface structures with proteolytic activity that degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
3.4K
The Tumor Microenvironment
8.0K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
8.0K