特定のmRNA結合因子は,シトプラズマのトランスファーリン受容体mRNAの鉄に依存する安定性を調節する

E W Müllner¹, B Neupert, L C Kühn

¹Swiss Institute for Experimental Cancer Research Genetics Unit, Epalinges, Switzerland.

Cell

|July 28, 1989

まとめ

細胞の鉄のホメオスタシスは,トランスファーリン受容体 (hTR) mRNAの安定性とフェリチン翻訳の両方を調節する共通の因子によって維持されます. この要素は,この因子です.

科学分野:

分子生物学は分子生物学である.
遺伝子規制遺伝子規制
鉄代謝について

背景:

鉄は細胞機能に不可欠であり,そのレベルは厳格に規制されています.
人間のトランスファーリン受容体 (hTR) の発現は,鉄の供給によって調節される.
転写後の調節は,遺伝子発現を制御する上で重要な役割を果たします.

研究の目的:

鉄に依存するhTR mRNAの安定性の規制メカニズムを特定する.
hTR mRNAの調節に関与する細胞質素因子を特徴付ける.
鉄によるhTRとフェリチンmRNAの調節の関係を調査する.

主な方法:

細胞プラズマ因子を特定し,特徴づけるためのRNA結合測定法.
異なる鉄条件下でのmRNAの安定性の分析.
フェリチン mRNA 調節配列を用いた競争アッセイ.

主要な成果:

特定の細胞プラズマ因子は,hTR mRNA 3' 未翻訳領域の同類のパリンドロームと結合する.
鉄のケレーションにより,因子結合活性とhTR mRNAレベルが増加する.
鉄の添加は,hTR mRNAの分解に先立ち,素因子の活性を急速に低下させます.
この因子の結合はフェリチンmRNAの調節配列によって競争されます.

結論:

共通の細胞プラズマ因子は,鉄に依存するhTRとフェリチン発現の調節を調整する.
この因子は,hTR mRNAの安定性の転写後の制御を媒介する.
細胞の鉄のホメオスタシスは,鉄の吸収 (hTR) と貯蔵/利用 (フェリチン) の調整された調節によって維持されます.

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