SEDSタンパク質は,細菌の細胞壁ポリマーゼの一種である
Alexander J Meeske1, Eammon P Riley1, William P Robins1
1Department of Microbiology and Immunobiology, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA.
Nature
|August 16, 2016
まとめ
バシルス・サブティリスでは,ロッド複合体は,既知のポリメラーゼなしで細胞壁を合成する. この研究は,SEDSタンパク質が重要なペプチドグライカンポリメラーゼであり,細菌の成長と潜在的な抗生物質標的に不可欠であることを明らかにしています.
科学分野:
- 微生物学
- 分子生物学
- 生物化学
背景:
- バクテリアの棒状細胞の伸びは,ペプチドグリカン合成のためのRod複合体に依存しています.
- 細胞壁生殖におけるSEDS (形状,延伸,分裂および胞子形成) タンパク質の正確な機能は不明である.
研究 の 目的:
- バクテリアの細胞壁合成における SEDS タンパク質の役割を調査する.
- SEDSタンパク質がペプチドグリカンポリメラーゼとして機能するかどうかを判断する.
主な方法:
- バチルス・サブティリスの遺伝子解析
- SEDSタンパク質の生化学的特徴
主要な成果:
- ロッド複合体は,ペプチドグリカンポリメラーゼが知られていない場合でもバシルス・サブティリスで機能する.
- 既知のポリメラーゼが欠けている細胞は,エンベロープのストレス反応を通じて,SEDSタンパク質であるRodaを調節する.
- 遺伝的および生化学的なデータは,SEDSタンパク質がペプチドグリカンポリメラーゼであることを示しています.
結論:
- バクテリアは,エクソスケルトン合成のために2つの異なるペプチドグリカンポリメラーゼを使用します.
- SEDSタンパク質は,バクテリアの伸びと分裂に不可欠なコア細胞壁合成です.
- SEDSタンパク質は 新種の抗生物質の開発において有望な標的となる.
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