細胞環境によるナノファイバーの自己組み立て
Zhen Zheng1, Peiyao Chen1, Maolin Xie1
1CAS Key Laboratory of Soft Matter Chemistry, †Department of Chemistry and ⊥Department of Polymer Science and Engineering and ‡Hefei National Laboratory for Physical Sciences at the Microscale, Department of Chemical Physics, University of Science and Technology of China , 96 Jinzhai Road, Hefei, Anhui 230026, China.
Journal of the American Chemical Society
|August 18, 2016
まとめ
この研究は,がん細胞内の2つの異なるナノファイバーに自己組み立て,標的の生物学的アプリケーションのための細胞外と細胞内環境を区別する新しい前駆体を提供します.
科学分野:
- バイオマテリアル科学
- 超分子化学
- ナノテクノロジー
背景:
- 細胞環境は 超分子自己組み立ての ユニークな機会を提供します
- 単一の先駆体から異なるナノファイバー形成のための細胞外と細胞内環境の区別は,依然として重要な課題である.
研究 の 目的:
- 異なる細胞環境 (細胞外と細胞内) に応じて2つの異なるナノファイバーを形成できる前駆体を設計する.
- ガン細胞におけるアルカリリンフォスファタゼ (ALP) とグルタチオン (GSH) の差異的な存在を活用して,自己組織化を行う.
- クリック化学を用いた超分子ナノファイバーの容易な後編成のためのプラットフォームを確立する.
主な方法:
- ALPとGSHに敏感な割れる結合を組み込む前駆体分子 (Cys(SEt) -Glu-Tyr ((H2PO3) -Phe-Phe-Gly-CBT) の合理的な設計.
- ALPとGSHで先駆体の一連の処理により,アンフィフィリックなナノファイバーに差異化および自己組み立てが誘発されます.
- クリックコンデンサ反応を利用して,組み立て後の修正を行う.
主要な成果:
- 設計された前身は,連続した酵素および化学処理で2つの異なるアンフィフィリック構造を成功裏に得られました.
- これらの構造は水素ゲル環境内で異なるナノファイバーに自己組み立てられ 環境への反応性を示しています
- クリック凝縮反応は形成されたナノファイバーの容易なポストモジュール化を可能にしました.
結論:
- 2つの異なるナノファイバーの連続的,環境特有の自己組み立てのための多機能な前駆体が開発されました.
- このアプローチは,がん細胞内の細胞外と細胞内の両方をターゲットにするための新しい戦略を提供します.
- このプラットフォームは先進的な生物学的応用と 薬物投与システムの可能性を秘めています
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