Search research articles

お問い合わせ

JoVEについて

概要リーダーシップブログ JoVEヘルプセンター

著者向け

出版プロセス編集委員会範囲と方針査読よくある質問投稿

図書館員向け

推薦の声購読アクセスリソース図書館諮問委員会よくある質問

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments アーカイブ

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教員リソースセンター教員サイト

プライバシーポリシー

関連する概念動画

Synthetic Biology

Synthetic Biology

Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...

Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.

DNA as a Genetic Template

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...

From DNA to Protein

From DNA to Protein

The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え

Same author

Manufacturing-aware generative models enable petascale synthesis of designed DNA.

Nature biotechnology·2026

Same author

SEED-Selection enables high-efficiency enrichment of primary T cells edited at multiple loci.

Nature biotechnology·2025

Same author

Ultra-high efficiency T cell reprogramming at multiple loci with SEED-Selection.

bioRxiv : the preprint server for biology·2024

Same author

Genetically corrected RAG2-SCID human hematopoietic stem cells restore V(D)J-recombinase and rescue lymphoid deficiency.

Blood advances·2023

Same author

Modular pooled discovery of synthetic knockin sequences to program durable cell therapies.

Cell·2023

Same author

Evolutionary constraint and innovation across hundreds of placental mammals.

Science (New York, N.Y.)·2023

Search research articles

関連する実験動画

Updated: Mar 16, 2026

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

Published on: May 25, 2015

57 コドンのゲノムの設計,合成,検査

Nili Ostrov¹, Matthieu Landon², Marc Guell³

¹Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.

Science (New York, N.Y.)

|August 20, 2016

まとめ

科学者は7つのコドンを再コードすることで 57のコドンのエシェリキア・コライのゲノムをコンピューターで設計し合成しましたほとんどの重要な遺伝子は機能を維持し,合成生物学の応用のためにゲノムを再記述する可能性を実証した.

さらに関連する動画

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

関連する実験動画

Last Updated: Mar 16, 2026

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

Published on: May 25, 2015

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

科学分野:

合成生物学
ゲノミクス
分子生物学

背景:

遺伝子コードの再コーディングは,新しいゲノム機能を設計するための方法を提供します.
エシェリキア・コライは遺伝子操作と合成生物学のモデル生物です

研究の目的:

57コドンの大腸菌ゲノムをコンピューターで設計・合成・組み立て
7つのコドンの62,214個を代名詞で置き換える
大規模な合成ゲノム工学の枠組みを確立する

主な方法:

すべてのタンパク質をコードする遺伝子の7つのコドンの計算式ゲノム設計と再コーディング.
3.97メガベースゲノムの化学合成と組み立て
再コードされた遺伝子とゲノムセグメントの機能テストによる検証
致命的な設計の例外を特定し修正する.

主要な成果:

エシェリキア・コライの全ゲノムで 6万2214個のコドンを再コードしました
再コードされた遺伝子の63%が検証され,重要な遺伝子の91%が機能を維持した.
2229の遺伝子の中で致命的なデザインの例外を特定し修正しました
基本的遺伝子に限られた適応効果を示した.

結論:

ゲノム再コーディングは機能が強化された合成生物を作るための実現可能な戦略です
この研究は,大規模合成ゲノムの設計,組み立て,分析のための堅固な枠組みを提供します.
この研究は新種の遺伝子コードを持つゲノムの構築を可能にすることで合成生物学の分野を前進させています