レチノール吸収のためのSTRA6受容体の構造
Yunting Chen1, Oliver B Clarke2, Jonathan Kim1
1Department of Physiology and Cellular Biophysics, Columbia University, New York, NY 10032, USA.
まとめ
ビタミンAのトランスポーターであるゼブラフィッシュのSTRA6の構造は,その二重構造とユニークなカルモジュリン結合部位を明らかにします. この発見は,リピド二層への直接拡散によるレチノールの吸収のための新しいメカニズムを示唆しています.
科学分野:
- 生物化学
- 構造生物学
- 細胞生物学
背景:
- ビタミンAのホメオスタシスは 細胞機能に不可欠です
- レチノール結合タンパク質 (RBP) は,ビタミンAの主な形態であるレチノールを輸送します.
- STRA6受容体は,RBP-レチノールと相互作用することで,細胞のビタミンA吸収を促進する.
研究 の 目的:
- 斑馬魚のSTRA6受容体の高解像度構造を決定する.
- RBP-レチノールの認識とビタミンAの吸収の構造的基礎を明らかにする.
主な方法:
- 単粒子の冷凍電子顕微鏡 (冷凍EM) を用いてゼブラフィッシュSTRA6の構造を決定した.
- 構造は3.9アングストロムの解像度でした.
主要な成果:
- 構造は,STRA6が1つの内膜と9つの外膜ヘリクスを有する複雑な二重組成であることを明らかにした.
- カルモジュリンは,STRA6に固く結合していることが判明しました.
- 主要なRBP結合残基は,膜に開いた深い脂性裂け目の上にアーチ状の構造を形成する.
結論:
- 二重構造とカルモジュリン相互作用は,STRA6の機能に関する洞察を提供します.
- リポフィルの裂け方は,リピド二層への直接的な拡散を通して,レチノールの内部化の可能性のあるメカニズムを示唆する.
さらに関連する動画
08:18Author Spotlight: Unraveling Vitamin A Transport Mechanisms — Linking Liver Receptors to Vision Health Through RBPR2 and RBP4 Interactions
Published on: October 4, 2024
1.6K
14:32Real-time Analyses of Retinol Transport by the Membrane Receptor of Plasma Retinol Binding Protein
Published on: January 28, 2013
14.2K
関連する概念動画
Receptor-mediated Endocytosis
112.9K
Overview
112.9K
Receptor-mediated Endocytosis
11.2K
Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
11.2K
Receptor-Mediated Endocytosis
5.6K
5.6K
Channel Rhodopsins
3.4K
Most organisms use photoreceptors to sense and respond to light. Examples of photoreceptors include bacteriorhodopsins and bacteriophytochromes in some bacteria, phytochromes in plants, and rhodopsins in the photoreceptor cells of the vertebral retina. The light-sensitive property of these receptors is because of the bound chromophores, such as bilin in the phytochromes and retinal in the rhodopsins.
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
3.4K
The Retina
78.3K
The retina is a layer of nervous tissue at the back of the eye that transduces light into neural signals. This process, called phototransduction, is carried out by rod and cone photoreceptor cells in the back of the retina.
78.3K
Anatomy of the Eyeball
11.2K
The eye is a spherical, hollow structure composed of three tissue layers. The outer layer — the fibrous tunic, comprises the sclera — a white structure — and the cornea, which is transparent. The sclera encompasses some of the ocular surface, most of which is not visible. However, the 'white of the eye' is distinctively visible in humans compared to other species. The cornea, a clear covering at the front of the eye, enables light penetration. The eye's middle...
11.2K
