関連する実験動画
Updated: Mar 15, 2026

08:39
Electroporation of Functional Bacterial Effectors into Mammalian Cells
Published on: January 19, 2015
10.5K
核孔浸透は,エフェクター誘発免疫における収束シグナルイベントである
Yangnan Gu1, Sophia G Zebell1, Zizhen Liang2
1Department of Biology, Howard Hughes Medical Institute-Gordon and Betty Moore Foundation, P.O. Box 90338, Duke University, Durham, NC 27708, USA.
Cell
|August 30, 2016
まとめ
核孔複合タンパク質CPR5は核輸送を制御することで 植物免疫を調節する. CPR5 について
科学分野:
- 植物免疫
- 分子生物学
- 細胞生物学
背景:
- 核輸送は免疫反応に不可欠です
- 核孔複合体 (NPC) による核輸送の制御は完全に理解されていません.
- エフェクタトリガー免疫 (ETI) とプログラム細胞死 (PCD) は,重要な植物防御メカニズムです.
研究 の 目的:
- 植物免疫反応における核輸送の制御における CPR5 の役割を調査する.
- 核孔複合体 (NPC) がエフェクター誘発免疫 (ETI) で機能するメカニズムを解明する.
主な方法:
- CPR5の相互作用相手を特定するための生化学的測定
- CPR5の局所化と動態を視覚化するためのコンフォカル顕微鏡.
- 植物免疫におけるCPR5機能の遺伝子解析
主要な成果:
- CPR5は,NPC選択バリアと関連した新しいトランスメブランヌクレオポリンです.
- CPR5はシグナリング分子の核侵入を抑制し,サイクリン依存キナーゼ阻害剤 (CKI) を隔離する.
- 免疫受容体の活性化により,CPR5の形状が変化し,CKIが放出され,核シグナル伝達が強化されるためにNPCの透過性が変化します.
結論:
- CPR5は,ETIの間,NPCで核輸送の重要なレギュレータとして機能します.
- CPR5のコンフォメーションスイッチは,堅固なETI/PCD誘導のためにCKIの解放とNPCの再構成を調整する.
- これらの発見は,NPC調節による免疫信号増幅の新しいメカニズムを明らかにしています.
関連する概念動画
Nuclear Export
5.1K
The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
5.1K
Regulation of Nuclear Protein Sorting
3.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
3.4K
Nuclear Protein Sorting
6.6K
Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
6.6K
Cells of the Innate Immune Response
10.2K
The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
10.2K
Nuclear Localization Signals and Import
8.1K
Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of 2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...
8.1K
Cytotoxic T Cells-mediated Immune Response
7.8K
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
7.8K

