sRNA媒介によるE. coliにおける転写終結の制御

Nadezda Sedlyarova¹, Ilya Shamovsky², Binod K Bharati³

¹Department of Biochemistry and Cellbiology, Max F. Perutz Laboratories, University of Vienna, Dr. Bohrgasse 9/5, 1030 Vienna, Austria; Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, NY 10016, USA.

Cell

|September 24, 2016

まとめ

バクテリアの小さなRNA (sRNA) は,早めの転写終結を防ぐことで遺伝子発現を調節する. rpoS遺伝子で実証されたこのメカニズムは,環境の変化に対する細菌の適応を高めます.

科学分野:

分子生物学
バクテリア遺伝学
遺伝子規制

背景:

バクテリアの小さなRNA (sRNA) は,転写後の遺伝子発現のレギュレータとして知られています.
rpoS遺伝子は,細菌の適応に不可欠な一般的なストレスシグマ因子をコードする.
Rho依存転写終結は遺伝子発現を制御する重要なプロセスである.

研究の目的:

バクテリアの転写終了における sRNA の役割を調査する.
sRNAがRho依存端末を調節できるかどうかを判断する.
rpoS遺伝子発現に対するsRNA媒介終結制御の影響を理解する.

主な方法:

sRNAとRpoSの相互作用を研究するための in vitroおよびin vivoアッセイ.
rpoS遺伝子をモデルとして用いた転写終結の分析.
次の世代のRNA配列とRho機能のバイオ情報分析

主要な成果:

sRNAs DsrA,ArcZ,およびRprAは,rpoS 5'UTRに結合し,早期のRho依存終結を抑制する.
sRNA媒介によるアンチターミネーションは,静止期におけるrpoS転写を著しく増加させる.
Rhoは,多数の細菌遺伝子の5'UTRでグローバルトランスクリプション減衰剤として作用する.

結論:

sRNAは細菌における転写終結の新たな調節体である.
sRNAによるRho依存終結の抑制は,広範な調節メカニズムである.
この調節経路は代謝や環境の変化にバクテリアの迅速な適応を促します

キーワード:

ロートランスクリプションの終了アンチターミネーション sRNA についてシグマ・ファクターストレス反応

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