CRISPR-C2c2の2つの異なるRNase活動は,ガイドRNA処理とRNA検出を可能にします
Alexandra East-Seletsky1, Mitchell R O'Connell1, Spencer C Knight2
1Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA.
Nature
|September 27, 2016
まとめ
バクテリアのC2c2タンパク質には2つの異なるRNA分裂活動があります. 1つは免疫監視のためにCRISPRRNAを成熟させ,もう1つはRNAを分解し,敏感なトランスクリプト検出を可能にします.
科学分野:
- 分子生物学
- 微生物学
- 生物化学
背景:
- CRISPR-Casシステムは 細菌に適応免疫を与えます
- C2c2を含むタイプVIのCRISPRシステムは,ターゲットRNA基板である.
- crRNAの成熟とC2c2によるRNA分裂のメカニズムは以前は知られていなかった.
研究 の 目的:
- バクテリアのC2c2タンパク質の二重RNase活性を解明する.
- C2c2が成熟したCRISPRRNA (crRNA) を取得する方法を理解する.
- C2c2によるcrRNA媒介RNA分裂のメカニズムを調査する.
主な方法:
- C2c2のRNase活動を特徴付けるための生化学的測定.
- Cpf1のような他のCRISPR酵素との比較分析
- C2c2のRNase機能の独特の化学的および機械的性質を調査する.
主要な成果:
- バクテリアのC2c2は,CRISPR RNAの成熟のためのユニークなRNase活性を示しています.
- C2c2は独立したRNA活性化単一鎖RNA分解活性を持っています.
- これらの二重RNase機能は,化学的および機械的に異なる.
結論:
- C2c2の二重RNase活動は,ガイドRNAの多重処理とロードを可能にします.
- このメカニズムは 細胞トランスクリプトの 敏感な検出を可能にします
- C2c2はCRISPR RNAの処理と機能の異なる経路を表しています.
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