細胞機能の配線図をマッピングするグローバル遺伝子相互作用ネットワーク
Michael Costanzo1, Benjamin VanderSluis2, Elizabeth N Koch3
1The Donnelly Centre, University of Toronto, 160 College Street, Toronto ON, Canada M5S 3E1.
まとめ
研究 者 たち は 酵母 菌 サッカロマイセス ・ セレヴィシア の 地図 を 作成 し まし た
科学分野:
- * システム生物学とゲノミクス
- * 分子生物学と細胞生物学
- * 酵母遺伝学
背景:
- * 遺伝子の機能と細胞の組織を理解することは,生物学的研究にとって極めて重要です.
- * 遺伝子の相互作用は,遺伝子関係や生物学的経路についての洞察を提供します.
- * 以前の研究では,より小規模な遺伝子ネットワークをマッピングしました.
研究 の 目的:
- * サッカロミケス・セレヴィシア (Saccharomyces cerevisiae) のための包括的な世界的な遺伝子相互作用ネットワークを構築する.
- * 遺伝子の相互作用を分析することによって,細胞の機能的配線をマッピングする.
- * 遺伝子プロファイルに基づく細胞機能の階層モデルを開発する.
主な方法:
- * サッカロマイセ・セレヴィシアの2300万種以上のダブルミュータントの生成
- * ネガティブとポジティブの遺伝子相互作用を体系的に特定する
- * 細胞機能の階層的なモデルを組み立てるための生物情報分析
主要な成果:
- * 約55万のネガティブと35万の陽性遺伝子の相互作用を特定した.
- * ネットワーク内の密集したハブとして識別された重要な遺伝子.
- * 複合体,経路,プロセス,コンパートメントのためのモジュールを含む細胞機能の階層的なモデルの構築.
- * ネガティブな相互作用は,機能的に関連した遺伝子をリンクし,コアバイオプロセスをマップします.
- * ポジティブな相互作用は,一般的な規制関連性を示します.
結論:
- * 細胞の機能的な配線図を 提供している.
- * 遺伝的相互作用プロファイルは複雑な細胞機能をモデル化するのに強力です.
- * このネットワークは,遺伝子機能と細胞組織を理解するための貴重なリソースとして機能します.
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