分子モーターの力誘発方向スイッチは,並列の微小管束の形成を可能にします

Maxim I Molodtsov¹, Christine Mieck², Jeroen Dobbelaere³

¹Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, 1030 Vienna, Austria; Max F. Perutz Laboratories, University of Vienna, Dr. Bohr-Gasse 9, 1030 Vienna, Austria; Research Platform Quantum Phenomena & Nanoscale Biological Systems (QuNaBioS), Dr. Bohr-Gasse 7, 1030 Vienna, Austria.

Cell

|October 8, 2016

まとめ

EB1とキネシン-14モータータンパク質の保存されたシステムは,微小管の成長を導く. このメカニズムは細胞機能に不可欠な並列の微小管束を生成します

科学分野:

細胞生物学
分子モーター
細胞骨格の動力学

背景:

微小管組織センター (MTOC) は微小管の成長を開始します.
協調的な成長は,並列の微小管束のために必要ですが,そのメカニズムは不明です.

研究の目的:

一つのMTOCから並列微小管の成長を調整するメカニズムを解明する.
微小管の組織に関与する重要な分子プレーヤーを特定する.

主な方法:

EB1 (プラスエンドトラッカー) とキネシン-14 (マイナスエンドモーター) の役割を調査した.
イースト,ドロソフィラ,ヒト細胞を含むモデルシステムを活用した.
微小管のダイナミクスと運動タンパク質の振る舞いを分析した

主要な成果:

EB1とキネシン-14は,平行成長に十分な保存された2つの成分システムを形成することを実証しました.
示されたキネシン-14は,既存のマイクロチューブルに沿って成長するマイクロチューブルプラスエンドを導きます.
微小管の末端からの力によって運動の方向性が変化することを明らかにしました

結論:

保存されたEB1-キネシン-14システムは,並列の微小管組織を駆動する.
このメカニズムは,スピンドル組立や偏分化などの細胞機能に不可欠な並列マイクロチューブルネットワークの形成を説明します.

キーワード:

Cik1-Kar3 について EB1 についてパラレルマイクロチューブル方向スイッチキネシン-14 微小管のポリメリゼーションプラスエンドガイド分極化

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