18種の真菌におけるタンパク質リン酸化の進化
Romain A Studer1, Ricard A Rodriguez-Mias2, Kelsey M Haas2
1European Molecular Biology Laboratory (EMBL), European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SD, UK.
まとめ
タンパク質のリン酸化は 細胞の重要な信号伝達メカニズムで 菌類の間で急速に進化しています 古代のフォスフォサイトは新しいものより保存され,機能的に重要であり,現象的多様性を駆り立てる.
科学分野:
- 分子生物学
- 進化生物学
- 生物化学
背景:
- タンパク質のリン酸化は,細胞の信号伝達とタンパク質の機能を調節する重要な翻訳後の変化です.
- 生物学的複雑性におけるその役割を解読するには,リン酸化の進化動態を理解することが不可欠です.
研究 の 目的:
- 様々な真菌種における タンパク質フォスフォサイトの進化史を調査する.
- フォスフォシトの進化における保存と分散のパターンとその機能的影響を特定する.
主な方法:
- 18種のキノコの相対的なフォスフォプロテオミクス
- フォスフォサイトの進化軌跡を推論する 系統遺伝分析
- 保存されたおよび新たに取得されたフォスフォサイトの機能的濃縮分析.
主要な成果:
- フォスフォプロテオームの高度な分散は,長い進化の時間尺度で保存された少数のフォスフォサイトがある.
- 古代のフォスフォサイトは主にタンパク質の接点に位置し,機能的に有意である.
- リン酸化モチーフの頻度とキナーゼ活性における有意なシフトは,全ゲノム複製イベントと相関する.
結論:
- この研究は,タンパク質のフォスフォサイト動態を理解するための進化的枠組みを提供します.
- リン酸化の急速な進化は,真核生物の表型多様性の発生に大きく貢献する.
- 保存されたフォスフォサイトは 細胞核のプロセスにおいて 根本的な役割を果たす可能性が高い.
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