エリソイド特異的転写因子Eryf1:新しい指タンパク質である

T Evans¹, G Felsenfeld

¹Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892.

Cell

|September 8, 1989

まとめ

赤い血球の発達のマスター・スイッチとして作用するエリフ1というエリソイド特異的転写因子. この研究は,Eryf1を特定しています.

科学分野:

分子生物学は分子生物学である.
発達生物学発達生物学とは
遺伝学遺伝学とは

背景:

エリソイドの発達には,複雑な遺伝子調節が含まれています.
転写因子は細胞の分化において重要な役割を果たします.
エリソイド系へのコミットメントを制御する特定の要因は,完全に理解されていません.

研究の目的:

エリホイドに特異的な転写因子Eryf1.1を特定し,特徴づけること.
グロービン遺伝子発現の調節におけるEryf1の役割を明らかにする.
Eryf1のDNA結合の分子メカニズムを理解するために.

主な方法:

Eryf1 cDNAのクローニング.
様々な細胞系におけるmRNA発現分析.
Eryf1の結合活性を実証した.
モチーフ分析を含むタンパク質の特徴化.

主要な成果:

Eryf1 mRNAは,赤色素細胞にのみ存在します.
クローンされたEryf1cDNAは,特定のDNA結合活動をコードする.
Eryf1は38 kDaの基本的なタンパク質で,独特のシステインに富んだ指のモチーフを持っています.
Eryf1のDNA結合には,移行金属イオンを必要としない場合があります.

結論:

Eryf1は,赤色球体の発達の重要なレギュラーである.
Eryf1は,アルファおよびベータグロービン遺伝子ファミリーの両方に一般的なスイッチ因子として作用します.
Eryf1のユニークな構造は,DNA結合の新たなメカニズムを示唆しています.

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