二酸化炭素を in vitro で固定するための合成経路
Thomas Schwander1, Lennart Schada von Borzyskowski1,2, Simon Burgener1,2
1Biochemistry and Synthetic Biology of Microbial Metabolism Group, Max Planck Institute for Terrestrial Microbiology Marburg, D-35043 Marburg, Germany.
Science (New York, N.Y.)
|November 19, 2016
まとめ
研究者は,二酸化炭素 (CO2) を有機分子に変換する合成クロトニル-コエンザイムA/エチルマロニル-CoA/ヒドロキシブチリル-CoA (CETCH) サイクルを開発しました. この新しい人工経路はグリーンエコノミーにおける 炭素固定の新たな戦略を提供します
科学分野:
- 合成生物学
- 生物化学
- 緑の化学
背景:
- 二酸化炭素 (CO2) は持続可能なグリーン経済の重要な原料です.
- CO2を価値ある多炭素化合物に効率的に変換することが重要です.
- 既存の天然のCO2固定経路は限られている.
研究 の 目的:
- 継続的な in vitro 二酸化炭素固定のための効率的な合成サイクルを開発する.
- 二酸化炭素を有機分子に変換する 新しい人工経路を作り出すこと
- 自然に進化した6つの経路を超えて第7のCO2固定経路を確立する.
主な方法:
- 代謝逆合成は,クロトニル-共酵素A/エチルマロニル-CoA/ヒドロキシブチリル-CoA (CETCH) サイクルを設計するために使用された.
- 生命の3つの領域にまたがる様々な生物から 17の酵素のネットワークを構成しています
- 酵素工学と代謝校正が最適化のために使用された.
主要な成果:
- 合成CETCHサイクルでは CO2を有機分子に連続的に固定します
- 1ミリグラムのタンパク質に 1分間に5ナノモルのCO2を排出します
- この経路は 9種類の酵素を用いて成功しました
結論:
- CETCHサイクルは新しい第7のCO2固定経路を表しています.
- この合成経路は,in vitroおよびin vivoの応用のための新しいツールを提供します.
- 開発はグリーンエコノミーにおける炭素の吸収と利用の戦略を進めている.
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