Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Recycling Endosomes and Transcytosis00:58

Recycling Endosomes and Transcytosis

3.7K
The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route.
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
3.7K
The Early Endosome: Endocytosis of Transferrin01:28

The Early Endosome: Endocytosis of Transferrin

5.0K
Essential proteins such as insulin or low-density lipoprotein (LDL) and micronutrients such as iron enter a eukaryotic cell through receptor-mediated endocytosis. Subsequently, the early endosomes fuse with the vesicles containing such receptor-ligand complexes and play a vital role in sorting the incoming ligands and receptors. While the ligands are either degraded inside the vesicle or released into the cytosol, their receptors are returned to the plasma membrane for further rounds of...
5.0K
Export of Misfolded Proteins out of the ER01:32

Export of Misfolded Proteins out of the ER

5.4K
After folding, the ER assesses the quality of secretory and membrane proteins. The correctly folded proteins are cleared by the calnexin cycle for transport to their final destination, while misfolded proteins are held back in the ER lumen. The ER chaperones attempt to unfold and refold the misfolded proteins but sometimes fail to achieve the correct native conformation. Such terminally misfolded proteins are then exported to the cytosol by ER-associated degradation or ERAD pathway for...
5.4K
ER Retrieval Pathway01:45

ER Retrieval Pathway

5.0K
In the secretory pathway, vesicles transport proteins from one cellular compartment to another in forward transport to deliver the protein to its correct location. Occasionally, misfolded proteins and incorrect proteins escape their original compartments, and a retrieval pathway is used to return the escaped proteins to their original compartment.
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
5.0K
Rab Cascades01:25

Rab Cascades

3.7K
Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
3.7K
Translocation of Proteins into the Mitochondria01:19

Translocation of Proteins into the Mitochondria

13.6K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
13.6K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Clathrin-independent endocytosis and retrograde transport in cancer cells tune immune synapse organization and CD8 T cell response.

eLife·2026
Same author

From molecular mechanisms of glycosphingolipid function to bio-therapeutic applications.

Cellular and molecular life sciences : CMLS·2026
Same author

The Gb3-synthase A4GALT is an epigenetically regulated driver of tumor invasiveness in gastrointestinal cancer.

BMC cancer·2026
Same author

[Growth factor-triggered de-sialylation controls breast cancer invasion].

Medecine sciences : M/S·2026
Same author

Membrane glycoproteins get another go: the GlycoSwitch.

Trends in cell biology·2025
Same author

Spatial N-glycan rearrangement on α<sub>5</sub>β<sub>1</sub> integrin nucleates galectin-3 oligomers to determine endocytic fate.

Nature communications·2025
Same journal

A viral ORFeome library for systems-level genetic dissection of host-pathogen interactions.

Cell·2026
Same journal

Co-option of lysosomal machinery shapes the evolution of the intracellular photosymbiosis supporting coral reefs.

Cell·2026
Same journal

LEF1 and niche factors determine T cell stemness across chronic diseases.

Cell·2026
Same journal

Recurrent patterns of TOP1-mediated neuronal genomic damage shared by major neurodegenerative disorders.

Cell·2026
Same journal

Four-dimensional molecular mapping from a spatial snapshot reveals the dynamics of hair follicle organogenesis.

Cell·2026
Same journal

Whole-cell particle-based digital twin simulations from 4D lattice light-sheet microscopy data.

Cell·2026
関連記事をすべて見る

関連する実験動画

Updated: Mar 11, 2026

Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells
11:05

Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells

Published on: February 21, 2019

9.7K

レトロメア が エンドソーマ の 荷物 分類 に 罠 を 設ける

Ludger Johannes1, Christian Wunder1

  • 1Institut Curie, PSL Research University, Chemical Biology of Membranes and Therapeutic Delivery unit, INSERM U 1143, CNRS UMR 3666, 26 rue d'Ulm, 75248 Paris Cedex 05, France.

Cell
|December 3, 2016
PubMed
まとめ
この要約は機械生成です。

レトロマー複合体は 内分泌体から 膜の輸送を促します ルーカスと仲間たち レトロメアのサブユニットが 細胞内膜を駆動し 荷物を認識する方法を明らかにします

さらに関連する動画

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking
08:51

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking

Published on: February 12, 2022

4.0K
Live-cell Imaging of Endocytic Transport using Functionalized Nanobodies in Cultured Cells
08:02

Live-cell Imaging of Endocytic Transport using Functionalized Nanobodies in Cultured Cells

Published on: October 17, 2025

1.0K

関連する実験動画

Last Updated: Mar 11, 2026

Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells
11:05

Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells

Published on: February 21, 2019

9.7K
The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking
08:51

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking

Published on: February 12, 2022

4.0K
Live-cell Imaging of Endocytic Transport using Functionalized Nanobodies in Cultured Cells
08:02

Live-cell Imaging of Endocytic Transport using Functionalized Nanobodies in Cultured Cells

Published on: October 17, 2025

1.0K

科学分野:

  • 細胞生物学
  • 分子生物学
  • 生物化学

背景:

  • 細胞内の分子の移動を伴う 細胞機能には 膜の輸送が不可欠です
  • レトロマー複合体は,エンドソームからトランス・ゴルギネットワーク,およびエンドソームからプラズマ膜輸送の重要な媒介体である.
  • 機能不全のレトロマーが 神経変性疾患と関連しており,その重要性を強調しています.

研究 の 目的:

  • レトロマー媒介の膜取引の構造的メカニズムを解明する.
  • レトロマー複合体の認識と結合を 理解する
  • レトロマーによる膜採用と貨物認識の統合を調査する.

主な方法:

  • クリオ電子顕微鏡を用いた荷重結合レトロマー複合体の構造分析.
  • レトロマーサブユニットと貨物の間のタンパク質相互作用を研究する生化学的測定法.
  • レトロマー複合体の組成と機能の in vitro 再構成

主要な成果:

  • 荷物に結合した レトロマー複合体の 詳細な構造の洞察
  • 膜結合を媒介するレトロマーサブユニット間の協力的相互作用の特定.
  • 貨物の認識が膜の採用と結びつくメカニズムの特徴.
  • この研究は,レトロマーサブユニットが一緒に働き,小胞の形成を開始する方法を示しています.

結論:

  • レトロマー複合体は,エンドソーム膜の採用と荷物の選択のための協力的メカニズムを使用します.
  • 構造データは,膜取引におけるレトロマー機能のメカニズム的理解を提供します.
  • この研究は,レトロマー関連疾患の潜在的な治療目標に関する洞察を提供します.