設計されたタンパク質は,ナノケージを含む細胞外小胞の形成を誘導する
Jörg Votteler1, Cassandra Ogohara2,3, Sue Yi2,3
1Department of Biochemistry, University of Utah, Salt Lake City, Utah 84112, USA.
Nature
|December 6, 2016
まとめ
科学者たちは,ヒトの細胞から自己組織化して芽生える 新種のタンパク質ナノケージ (EPN) を設計した. これらのモジュラーなナノマテリアルは ウイルスの機能を真似て 細胞間の分子を運ぶことができます
科学分野:
- 合成生物学
- ナノテクノロジー
- 生物化学
背景:
- 複雑な生物学的機能は,ライボソームやウイルスなどの自己組織化マクロ分子ナノ構造に依存しています.
- 核酸またはタンパク質の自己組み立て構造の設計が確立されていますが,ハイブリッド材料工学が登場しています.
研究 の 目的:
- 膀内での細胞内放出を可能にする自己組織化タンパク質ナノケージを設計する.
- 細胞間輸送用の 遺伝子コード付きナノマテリアルを作る
主な方法:
- 膜結合,自己組み立て,ESCRT採用のための特定の配列要素を持つ合成タンパク質.
- 生物化学分析と冷凍電子顕微鏡を用いて ナノケージを特徴づけました
- 細胞融合と荷物の配送を可能にする ウイルスのグリコタンパク質を組み込みました
主要な成果:
- タンパク質ナノケージ (EPN) を開発し, 膀内のヒト細胞から芽を出す.
- 様々な合成タンパク質における EPN 設計戦略のモジュラリティと汎用性を実証した.
- 設計された60サブユニットタンパク質ナノケージを含む~100nmの膀を示す EPN-01.
- EPNが標的細胞と融合し 分子積荷を運ぶ能力を示した
結論:
- タンパク質は複雑な機能を持つ ハイブリッド生物学的物質を作るように プログラムすることができます
- EPNは設計された,モジュール化された,遺伝子組み換えナノマテリアルの新しいクラスです.
- EPNは細胞間分子移転を促進し,新しいバイオテクノロジーの応用の可能性を提供します.
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