Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Termination of Translation01:44

Termination of Translation

28.4K
The large ribosomal subunit has several important structures essential to translation. These include the peptidyl transferase center (PTC) - which is the site where the peptide bond is formed - and a large, internal, water-filled tube through which the nascent polypeptide moves. This latter structure is called the Peptide Exit Tunnel, and it begins at the PTC and spans the body of the large ribosomal subunit. During translation, as the nascent polypeptide chain is synthesized, it passes through...
28.4K
Termination of Translation01:44

Termination of Translation

6.9K
6.9K
Transcription Attenuation in Prokaryotes02:42

Transcription Attenuation in Prokaryotes

18.8K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
18.8K
Translation in Prokaryotes01:29

Translation in Prokaryotes

2.2K
Prokaryote translation is a complex, highly coordinated process that converts genetic information from mRNA into functional proteins. It involves three stages: initiation, elongation, and termination, each facilitated by specific molecular components.Initiation of TranslationThe process begins with the assembly of the ribosomal subunits and initiation factors on the mRNA. In bacteria, the 30S ribosomal subunit recognizes the Shine-Dalgarno sequence in the mRNA, a conserved region upstream of...
2.2K
Improving Translational Accuracy02:07

Improving Translational Accuracy

15.3K
Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
15.3K
Improving Translational Accuracy02:07

Improving Translational Accuracy

3.7K
3.7K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

A cellular basis for the mammalian nocturnal-diurnal switch.

Science (New York, N.Y.)·2026
Same author

An Industry Perspective on Key Considerations for the ICH S13 Guidance: Nonclinical Safety Evaluations of Oligonucleotide-Based Therapeutics.

International journal of toxicology·2026
Same author

Clinical considerations for the treatment of patients with familial chylomicronemia syndrome using a hepatic-targeted <i>APOC3</i> antisense oligonucleotide.

American journal of preventive cardiology·2025
Same author

Atomic models of the Toxoplasma cell invasion machinery.

Nature structural & molecular biology·2025
Same author

Proteome-wide <i>in silico</i> screening for human protein-protein interactions.

bioRxiv : the preprint server for biology·2025
Same author

Axonemal dynein contributions to flagellar beat types and waveforms.

bioRxiv : the preprint server for biology·2025
Same journal

A native sulfur deposit in Gale crater, Mars.

Science (New York, N.Y.)·2026
Same journal

Coordinated demise of harmful algal blooms.

Science (New York, N.Y.)·2026
Same journal

Genetic effects put into context.

Science (New York, N.Y.)·2026
Same journal

Bacteria share proteins to survive antibiotics.

Science (New York, N.Y.)·2026
Same journal

Impacts shaped Earth's first continents.

Science (New York, N.Y.)·2026
Same journal

Erratum for the Report "Covalently bonded single-molecule junctions with stable and reversible photoswitched conductivity" by C. Jia <i>et al</i>.

Science (New York, N.Y.)·2026
関連記事をすべて見る

関連する実験動画

Updated: Mar 10, 2026

Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein
05:48

Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein

Published on: March 16, 2022

3.1K

ストップコドンなしの翻訳終結

Nathan R James1, Alan Brown1, Yuliya Gordiyenko1

  • 1Medical Research Council (MRC) Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, CB2 0QH, UK.

Science (New York, N.Y.)
|December 10, 2016
PubMed
まとめ
この要約は機械生成です。

バクテリアのリボソームは,メッセンジャーRNA (mRNA) の末端で停止することがあります. 代替リボソーム救出因子A (ArfA) は,放出因子2 (RF2) を採用することによって,これらのノンストップ複合体を救出します.

さらに関連する動画

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on TRO Approach
12:12

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on TRO Approach

Published on: March 12, 2017

10.3K
Xenopus laevis as a Model to Identify Translation Impairment
10:24

Xenopus laevis as a Model to Identify Translation Impairment

Published on: September 27, 2015

11.2K

関連する実験動画

Last Updated: Mar 10, 2026

Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein
05:48

Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein

Published on: March 16, 2022

3.1K
Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on TRO Approach
12:12

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on TRO Approach

Published on: March 12, 2017

10.3K
Xenopus laevis as a Model to Identify Translation Impairment
10:24

Xenopus laevis as a Model to Identify Translation Impairment

Published on: September 27, 2015

11.2K

科学分野:

  • 分子生物学
  • 構造生物学
  • バクテリア 遺伝学

背景:

  • リボソームはストップコドンがないメッセンジャーRNA (mRNA) で停滞し,問題のあるノンストップ複合体を形成する.
  • バクテリアでは,代替リボソーム救出因子A (ArfA) が,これらの停滞したリボソームを解消するために不可欠です.

研究 の 目的:

  • ArfAがバクテリアのノンストップリボソームを救う構造的メカニズムを解明する.
  • ArfAが解放因子2 (RF2) とリボソームとどのように相互作用するかを理解する.

主な方法:

  • 電子冷凍顕微鏡 (cryo-EM) を用いて高解像度構造を決定した.
  • 構造は,3"断片化されたmRNAでリボソームに結合したArfAを捕獲した.

主要な成果:

  • ArfAはリボソームのmRNAチャネルに結合し,欠けているストップコドンの代わりとして作用する.
  • ArfAは,放出因子2 (RF2) をコンパクトで,事前に適応した状態に特定します.
  • ArfAに結合したRF2形状は,トランスレーションの終結の一般的なメカニズムを示唆する.

結論:

  • ArfAは,ストップコドンを模倣し,RF2を勧誘することで,トランスレーションの終結を促進します.
  • ArfAによって誘発される可能性のあるRF2のコンフォームスイッチは,ペプチドの放出の鍵です.
  • この研究は,細菌におけるリボソーム救済とトランスレーション終結の保存メカニズムを示しています.