動的二次イオン質量スペクトロメトリープローブにおける原子再結合 脂質組成における距離:ナノメートルの化学レール
Frank R Moss1, Steven G Boxer1
1Department of Chemistry, Stanford University , Stanford, California 94305-5012, United States.
Journal of the American Chemical Society
|December 16, 2016
まとめ
この研究では,ナノスケールの脂質組織を膜で測定するための新しい安定性同位体ラベリング法が導入されています. この技術はナノスケールの相分離を明らかにし,生物学的膜組織のより正確なモデルを提供します.
科学分野:
- 膜生物物理学
- 表面科学
- 分析化学
背景:
- 生物学的な膜組織はナノスケールで発生し,特徴化に課題を投げかけます.
- 既存の方法は,脂質とタンパク質の動態と小さなドメインサイズを解決するために苦労しています.
- 細胞機能の解読には 膜の横の組織を理解することが重要です
研究 の 目的:
- ナノスケールでの脂質コロカライゼーションの測定のための新しい方法を開発する.
- モデル膜におけるナノスケールの相分離を調査する.
- 生物学的膜組織のより正確なモデルを提供するために.
主な方法:
- 炭素-13 (C) と窒素-15 (N) との安定同位体ラベルを使用した.
- 動的二次イオン質量スペクトロメトリー (SIMS) でナノSIMS機器を用いたレバレッジされた原子再結合.
- C13−N15イオン形成の効率を測定し,分子近接を推論した.
主要な成果:
- 脂質二層および単層におけるナノスケール脂質組織を測定するための新しい技術を示した.
- モデル膜システムにおけるナノスケールの相分離の直接的証拠を提供した.
- 数ナノメートルのスケールでの異質性の解像度を達成し,従来のイメージングの限界を下回る.
結論:
- 開発されたNanoSIMSベースの方法は,ナノスケールの膜組織を正確に測定します.
- モデル膜におけるナノスケールの相分離は,マイクロスケールの分離と比較して,生物学的膜の優れた表現を提供します.
- この技術は,化学的および生物学的システムにおける近距離の研究に広く適用できます.
さらに関連する動画
12:11Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
Published on: February 27, 2020
7.4K
09:38Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
Published on: November 26, 2013
14.7K
関連する概念動画
01:21Tandem Mass Spectrometry
2.8K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.8K
01:19Mass Analyzers: Common Types
1.7K
The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
1.7K
01:33Peptide Identification Using Tandem Mass Spectrometry
8.7K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.7K
01:19Mass Spectrometry: Overview
9.6K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
9.6K
01:13Mass Analyzers: Overview
1.9K
The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
1.9K
01:21Mass Spectrometry: Complex Analysis
2.0K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
2.0K