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Updated: Feb 8, 2026

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
09:05

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

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マルチプレックスされた単細胞CRISPRスクリーニングプラットフォームは,展開されたタンパク質応答の体系的な解剖を可能にします

Britt Adamson1, Thomas M Norman1, Marco Jost2

  • 1Department of Cellular & Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA; Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA 94158, USA; California Institute for Quantitative Biomedical Research, University of California, San Francisco, San Francisco, CA 94158, USA; Center for RNA Systems Biology, University of California, San Francisco, San Francisco, CA 94158, USA.

Cell
|December 17, 2016
PubMed
まとめ

Perturb-seqは,単細胞RNA配列とCRISPRの混乱を組み合わせることで,深い機能的ゲノミクスを可能にします. この方法は,展開されたタンパク質応答 (UPR) のような複雑な細胞反応を高精度で解剖します.

キーワード:
クリップスリCRISPRについて単細胞RNA-seq細胞間異質性ゲノムスケールスクリーニング単細胞ゲノム解き放たれたタンパク質反応

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