Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Bacterial Protein Maturation01:26

Bacterial Protein Maturation

651
Bacterial protein maturation is a tightly regulated process that ensures newly synthesized polypeptides achieve correct functional conformations. This maturation involves a series of modifications, folding events, and quality control steps, often assisted by specialized chaperone proteins.N-Terminal ModificationsThe maturation of bacterial polypeptides begins cotranslationally as the polypeptide exits the ribosome. The first amino acid, N-formylmethionine (fMet), is typically modified at the...
651
Hyperthermophilic Bacteria01:21

Hyperthermophilic Bacteria

661
Domain Bacteria includes some unique hyperthermophilic species. They exhibit remarkable adaptations that enable survival in extreme environments.Thermotoga species are rod-shaped, gram-negative, non-sporulating hyperthermophiles that form a sheath-like envelope called a toga. They ferment sugars or starch, producing lactate, acetate, CO₂, and H₂, and can also grow via anaerobic respiration using H₂ and ferric iron. Found in hot springs and hydrothermal vents, over 20% of their...
661
Other Stress Responses in Bacteria01:30

Other Stress Responses in Bacteria

483
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
483
Diversity of Archaea III01:27

Diversity of Archaea III

410
Crenarchaeota, a prominent phylum of Archaea, is remarkable for its ability to thrive in extreme environments characterized by high temperatures and acidity. These microorganisms inhabit sulfuric hot springs, volcanic systems, and submarine hydrothermal vents, where temperatures often exceed 100°C. The unique adaptations of Crenarchaeota not only allow survival under such extreme conditions but also provide insights into the mechanisms of life in primordial Earth-like...
410
Diversity of Archaea IV01:29

Diversity of Archaea IV

569
Hyperthermophilic archaea are a group of extremophiles thriving at temperatures above 80°C, often in hydrothermal vents and volcanic soils where conditions surpass the boiling point of water. At such temperatures, proteins, membranes, and DNA in most organisms degrade, but hyperthermophiles have evolved remarkable adaptations to maintain stability and function.Unique Cellular FeaturesHyperthermophilic membranes are composed of a monolayer of biphytanyl tetraether lipids, which resist...
569
Cytoskeletal Proteins in Bacteria01:29

Cytoskeletal Proteins in Bacteria

4.4K
Bacterial cells were initially considered simple, randomly organized structures lacking a cytoskeleton. However, the discovery of cytoskeleton homologs in bacteria led to the change of this opinion. Bacterial cytoskeletal filaments regulate the cell shape, cell polarity, cell division, and partitioning of plasmids during cell division. It was later discovered that bacterial cytoskeletal proteins, mainly actin and tubulin homologs, are diverse compared to their eukaryotic counterparts. On the...
4.4K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

American science at 250.

Science (New York, N.Y.)·2026
Same author

Scalable assay to identify inhibitors of prion-like propagation of protein misfolding as potential therapeutics for neurodegeneration.

Protein science : a publication of the Protein Society·2026
Same author

Amide <sup>1</sup>H and <sup>15</sup>N NMR signal assignments of all naturally-occurring di-ubiquitins.

Biomolecular NMR assignments·2026
Same author

Selective control of ligand binding through a distal mutation that alters the protein native ensemble.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Local and Global Breathing Motions Prime the Access to Buried Binding Site in an Antibiotic-Sequestering Protein.

ACS bio & med chem Au·2025
Same author

Virus Propagation Linked to Exceedingly Rare Gene-Expression Errors: A Single-Molecule Microscopy Demonstration.

ACS chemical biology·2025

関連する実験動画

Updated: Mar 9, 2026

X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050
11:27

X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050

Published on: May 13, 2020

4.4K

バクテリアの熱センサータンパク質における段階的な構造的多形性

Abhishek Narayan1, Luis A Campos2, Sandhya Bhatia3

  • 1Department of Biotechnology, Bhupat & Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras (IITM) , Chennai 600036, India.

Journal of the American Chemical Society
|December 20, 2016
PubMed
まとめ
この要約は機械生成です。

病原菌は熱感を駆使して 毒性を制御します 研究者達はCnuタンパク質を研究し その第4のヘリックスが 熱感受モジュールとして作用し 細菌の温度適応と 毒性の遺伝子調節に 極めて重要です

さらに関連する動画

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
14:25

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

Published on: December 12, 2017

19.0K
Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues
07:08

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues

Published on: July 14, 2015

7.8K

関連する実験動画

Last Updated: Mar 9, 2026

X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050
11:27

X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050

Published on: May 13, 2020

4.4K
Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
14:25

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

Published on: December 12, 2017

19.0K
Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues
07:08

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues

Published on: July 14, 2015

7.8K

科学分野:

  • 分子生物学
  • バイオ物理学
  • 微生物学

背景:

  • 熱感は病原菌にとって 熱血宿主における 毒性の遺伝子発現を制御するために不可欠です
  • エントロバクテリアの一族のHhaタンパク質は,温度変化に反応して遺伝子発現を調節することが知られている.
  • 温度変化がタンパク質の構造と機能の変化に変換される方法については,分子レベルでの理解は限られている.

研究 の 目的:

  • 推定細菌の熱センサーであるCnuタンパク質の構造動態と分子機構を解明する.
  • 温度変化が分子レベルでCnuの構造と機能にどのように影響するか調査する.
  • Hhaタンパク質ファミリーの折りたたみ機能と信号伝達原理を理解する.

主な方法:

  • Cnuの形状的振る舞いを特徴づけるために,様々なスペクトロメトリック,カロメトリック,および水力動力学的測定を行いました.
  • 場所固有の光と核磁気共鳴 (NMR) 実験を使用した.
  • 統計的機械モデリングと分子動力学 (MD) シミュレーション (粗粒度および全原子) を含む複数のコンピューティングアプローチを統合した.

主要な成果:

  • Cnuは,探査に依存した展開,増加した構造的変動,および生理学的温度範囲内の温度依存の次元腫れを示し,可塑性のあるネイティブアンサンブルを示しています.
  • Cnuの4番目のヘリクスは 熱感受モジュールとして機能し,温度によって順序と方向の動的変化を示す.
  • 温度調節に対する熱感センサーモジュールの反応によって,連続的に展開する移行が観察されました.

結論:

  • この研究は,Cnu熱センサータンパク質の複雑な折り畳み機能の様子を明らかにしています.
  • Cnuの展開の複雑性と信号伝達における役割の分子の基礎を特定した.
  • タンパク質の折りたたみメカニズムとHhaファミリーの熱センサーの設計原理に対する機能的制約の影響を強調した.