関連する実験動画

Updated: Mar 9, 2026

Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement

Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement

Published on: July 26, 2017

mTORC1と筋肉再生は,LINC00961でコードされたSPARポリペプチドによって制御されます

Akinobu Matsumoto¹, Alessandra Pasut¹, Masaki Matsumoto²

¹Cancer Research Institute, Beth Israel Deaconess Cancer Center, Department of Medicine and Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA.

|December 27, 2016

まとめ

研究者らは,長い非コーディングRNA LINC00961によって暗号化された隠されたポリペプチド,SPARを発見しました. SPARはアミノ酸によるmTORC1の活性化を調節し,損傷後の筋肉再生に不可欠です.

科学分野:

分子生物学
遺伝学
細胞生物学

背景:

長い非コーディングRNA (lncRNAs) は,非コーディングの役割を超えた機能のためにますます認識されています.
lncRNAの中には,機能的なポリペプチドに変換できる小さなオープン・リーディングフレーム (ORF) がある.
lncRNAでコードされたこれらのポリペプチドの生物学的な意義は,ほとんど未知のままです.

研究の目的:

lncRNA LINC00961でコードされた新しいポリペプチドを識別し,機能的に特徴づける.
細胞信号伝達経路と組織再生におけるこのポリペプチドの役割を明らかにする.

主な方法:

LINC00961から新しいポリペプチド (SPAR) の識別と特徴付け.
SPARの局所化と相互作用 (例えばv-ATPase) を決定する生化学的測定法.
CRISPR/Cas9遺伝子編集により,SPAR特有のノックアウトマウスモデルが作成されます.
急性損傷後の筋肉再生を評価する in vivo 研究

主要な成果:

LINC00961から保存されたポリペプチド,SPARが特定され,後期エンドソーム/ライソームに局所されました.
SPARはv- ATPaseと相互作用し,アミノ酸刺激によるmTORC1の活性化を否定的に調節する.
SPARのノックアウトマウスは,mTORC1の活性化と損傷後の筋肉再生の改善を示した.
LncRNAのLINC00961発現は,骨格筋の損傷後のダウンレギュレーションです.

結論:

新種のlncRNAでコードされたポリペプチドであるSPARは,アミノ酸の可用性に応じてmTORC1の活性化を微調整するメカニズムを提供します.
この経路は損傷後の効率的な筋肉再生に不可欠です
lncRNAは小さなポリペプチドをコードし,組織特有の必要性のために基本的な生物学的プロセスを調節します.

さらに関連する動画

Minimally Invasive Muscle Embedding MIME - A Novel Experimental Technique to Facilitate Donor-Cell-Mediated Myogenesis

Minimally Invasive Muscle Embedding MIME - A Novel Experimental Technique to Facilitate Donor-Cell-Mediated Myogenesis

Published on: August 24, 2017

Author Spotlight: Exploring Orofacial Muscle Regeneration – Insights and Innovations

Author Spotlight: Exploring Orofacial Muscle Regeneration – Insights and Innovations

Published on: December 29, 2023

関連する概念動画

Formation of Muscle Fibers from Myoblasts

Formation of Muscle Fibers from Myoblasts

De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...

mTOR Signaling and Cancer Progression

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...

Master Transcription Regulators

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

Satellite Stem Cells and Muscular Dystrophy

Satellite Stem Cells and Muscular Dystrophy

Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...