人間のエストロゲン受容体には,2つの独立した非酸性転写活性化機能があります
1Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM Institut de Chimie Biologique, Faculté de Médecine, Strasbourg, France.
Cell
|November 3, 1989
まとめ
エストロゲン受容体 (ER) には2つの転写活性化機能があります:N端領域のTAF-1およびホルモン結合領域のTAF-2. TAF-1は,酸性活性化ドメインとは異なり,細胞特異的な活性を示しています.
科学分野:
- 分子生物学は分子生物学である.
- エンドクリノロジー エンドクリノロジー
- 遺伝子規制 遺伝子規制
背景:
- エストロゲン受容体 (ER) は,遺伝子調節において重要な役割を果たします.
- ERのホルモン結合領域におけるホルモン誘導型転写活性化機能 (TAF-2) を以前特定した.
研究 の 目的:
- 転写活性化機能のためのERのN端A/B領域を特徴付ける.
- TAF-1の特性をTAF-2および既知の酸活性化ドメイン (AADs) と比較する.
主な方法:
- ERのN末端領域 (TAF-1) によって異なった細胞タイプ (鶏の胚性線維芽細胞およびHeLa細胞) での転写活性化の分析.
- TAF-1,TAF-2,GAL4,およびVP16 AADsの間の相乗効果の相互作用を評価する.
- アデノウイルス2の主な遅発プロモーターのアップストリーム要素 (UE) 因子との相互作用を調査する.
主要な成果:
- ERのN-ターミナルA/B領域で構成的なアクティベーション関数 (TAF-1) を特定しました.
- TAF-1は細胞型特異性を有しており,鶏の胚線維芽細胞ではHeLa細胞よりも機能が優れている.
- TAF-1とTAF-2は,酸性アミノ酸ストレッチが欠けている酸性活性化ドメイン (AADs) に比べて,異なる活性化特性を有しています.
結論:
- エストロゲン受容体は,TAF-1 (構成性,細胞特異性) とTAF-2 (誘導性) の少なくとも2つの異なる転写活性化機能を備えています.
- TAF-1とTAF-2は,定規の酸性活性化ドメインと機械的に異なる.
- これらの発見は,エストロゲン受容体媒介遺伝子調節の複雑なメカニズムの理解に貢献します.
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