Wnt/β-cateninはマウスとヒトの胃底の特異性を促進する
Kyle W McCracken1, Eitaro Aihara2, Baptiste Martin1
1Division of Developmental Biology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio 45229, USA.
Nature
|January 5, 2017
まとめ
研究者は多能幹細胞からヒトのフンジック型胃器官 (hFGO) を開発した. これらのオルガノイドはヒトの胃底の発達と病気をモデル化し 薬剤発見のための新しいプラットフォームを提供します
科学分野:
- 幹細胞生物学
- 発達生物学
- 胃腸内科
背景:
- 胃疾患は世界的に蔓延しているが,ヒトの胃底の表皮には適切な研究モデルがない.
- 胃の病状の治療には fundus epitheliumの発達を理解することが重要です.
研究 の 目的:
- fundus epitheliumの発達と胃疾患を研究するためのヒトモデルを開発する.
- 骨盤上皮質の分化を制御する分子メカニズムを調査する.
主な方法:
- ヒトの多能幹細胞 (hPSC) は胃器官に微分化されました.
- 胚の底部発達における重要な出来事を特定し,概要をまとめました.
- Wnt/β-カテニンのシグナル伝達経路は,マウスの胚とhPSC由来の祖先で操作された.
主要な成果:
- hPSC由来前腸原産体におけるβ-カテニンのシグナリングの活性化により,ヒトのフンジック型胃器官 (hFGO) の発現が促進された.
- hFGOは,頭部と頭部細胞を含む fundus epitheliumを成功裏にモデル化しました.
- エピテリアの形態発生と分化における信号伝達経路の時間的に異なる役割が特定された.
結論:
- ヒトのフンディック型胃器官 (hFGO) は,ヒトの底部発達と胃疾患の研究に強力なモデルを提供します.
- hFGOはヒトの胃の生理学,病理生理学,薬剤発見の研究のための新しいプラットフォームを提供します.
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