Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Amyloid Fibrils03:03

Amyloid Fibrils

12.4K
Amyloid fibrils are aggregates of misfolded proteins.  Under most circumstances, misfolded proteins are either refolded by chaperone proteins or degraded by the proteasome. However, in the case of a mutation or a disease, these proteins can accumulate to form large clusters and often further assemble to form elongated fibers, called fibrils. 
Amyloid deposits were observed as early as 1639 in the liver and the spleen.   In 1854, Rudolph Virchow performed iodine staining,...
12.4K
Subviral Agents01:29

Subviral Agents

703
Subviral agents are infectious entities that resemble viruses but lack one or more viral components, such as a capsid or essential replication machinery. These agents include viroids, prions, and satellites, each possessing distinct structural and functional characteristics that influence their mode of infection and replication.Viroids are the simplest subviral agents, consisting of circular, single-stranded RNA molecules without a protein coat. They exclusively infect plants, relying entirely...
703
Bacterial Transformation01:33

Bacterial Transformation

61.8K
In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
61.8K
Bacterial Protein Maturation01:26

Bacterial Protein Maturation

651
Bacterial protein maturation is a tightly regulated process that ensures newly synthesized polypeptides achieve correct functional conformations. This maturation involves a series of modifications, folding events, and quality control steps, often assisted by specialized chaperone proteins.N-Terminal ModificationsThe maturation of bacterial polypeptides begins cotranslationally as the polypeptide exits the ribosome. The first amino acid, N-formylmethionine (fMet), is typically modified at the...
651
Bacterial Phylum Actinobacteria01:30

Bacterial Phylum Actinobacteria

782
Coryneform bacteria are gram-positive, aerobic, nonmotile rods that exhibit irregular, club-shaped, or V-shaped arrangements. Their V-shape results from snapping division, where the inner cell wall layer forms the cross-wall, while the outer layer remains intact until it ruptures on one side, causing the daughter cells to bend away.The primary genera are Corynebacterium and Arthrobacter. Corynebacterium includes diverse species, ranging from saprophytes to pathogens like Corynebacterium...
782
Bacterial Signaling01:30

Bacterial Signaling

42.5K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
42.5K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Minimal requirements for the epigenetic inheritance of engineered silent chromatin domains.

Proceedings of the National Academy of Sciences of the United States of America·2024
Same author

Measuring prion propagation in single bacteria elucidates a mechanism of loss.

Proceedings of the National Academy of Sciences of the United States of America·2023
Same author

Measuring prion propagation in single bacteria elucidates mechanism of loss.

bioRxiv : the preprint server for biology·2023
Same author

A Bacterial Cell-Based Assay To Study SARS-CoV-2 Protein-Protein Interactions.

mBio·2021
Same author

<i>Tombusvirus</i> p19 Captures RNase III-Cleaved Double-Stranded RNAs Formed by Overlapping Sense and Antisense Transcripts in Escherichia coli.

mBio·2020
Same author

Alternative transcription cycle for bacterial RNA polymerase.

Nature communications·2020

関連する実験動画

Updated: Mar 8, 2026

Protein Misfolding Cyclic Amplification of Prions
10:12

Protein Misfolding Cyclic Amplification of Prions

Published on: November 7, 2012

20.2K

バクテリアのグローバル・レギュレータがプリオンを形成する

Andy H Yuan1, Ann Hochschild2

  • 1Department of Microbiology and Immunobiology, Harvard Medical School, 4 Blackfan Circle, Boston, MA 02115, USA.

Science (New York, N.Y.)
|January 14, 2017
PubMed
まとめ
この要約は機械生成です。

バクテリアは自己増殖するタンパク質集合体であるプリオンを形成できる. 研究者達は プリオンを形成できる 細菌のタンパク質Rhoを発見し プリオンがユーカリ生物とバクテリアが 分裂する前に存在していたことを示唆しています

さらに関連する動画

Investigating the Spreading and Toxicity of Prion-like Proteins Using the Metazoan Model Organism C. elegans
12:57

Investigating the Spreading and Toxicity of Prion-like Proteins Using the Metazoan Model Organism C. elegans

Published on: January 8, 2015

16.6K
High-throughput Screening for Protein-based Inheritance in S. cerevisiae
08:12

High-throughput Screening for Protein-based Inheritance in S. cerevisiae

Published on: August 8, 2017

6.8K

関連する実験動画

Last Updated: Mar 8, 2026

Protein Misfolding Cyclic Amplification of Prions
10:12

Protein Misfolding Cyclic Amplification of Prions

Published on: November 7, 2012

20.2K
Investigating the Spreading and Toxicity of Prion-like Proteins Using the Metazoan Model Organism C. elegans
12:57

Investigating the Spreading and Toxicity of Prion-like Proteins Using the Metazoan Model Organism C. elegans

Published on: January 8, 2015

16.6K
High-throughput Screening for Protein-based Inheritance in S. cerevisiae
08:12

High-throughput Screening for Protein-based Inheritance in S. cerevisiae

Published on: August 8, 2017

6.8K

科学分野:

  • 微生物学
  • 分子生物学
  • タンパク質の生化学

背景:

  • プリオンは,自己増殖の集合体によって特徴づけられる,ユカリオットタンパク質ベースの遺伝要素として知られています.
  • プリオンは以前は細菌系で特定されていなかった.
  • プリオンの起源と分布を理解することは 進化生物学にとって極めて重要です

研究 の 目的:

  • 細菌のタンパク質のプリオン形成の可能性を調査する.
  • バクテリアのRho転写ターミネーター (Cb-Rho) のプリオン形成能力を特定し,特徴づけること.
  • バクテリアのプリオン発見の 進化的意味を探るため

主な方法:

  • Cb-Rho内の候補プリオン形成ドメイン (cPrD) の特定
  • 既知のプリオン領域 (PrD) を置き換えるcPrDの能力をテストする酵母における機能分析.
  • 溶解性および集積されたプリオン形態を含むEscherichia coliにおけるCb-Rho構成の表現と特徴づけ
  • ゲノム全体の遺伝子発現に対するCb-Rhoプリオン形態の影響を評価するためのトランスクリプトミックの分析.

主要な成果:

  • バクテリアのタンパク質Cb-Rhoはプリオンを形成する能力があると特定された.
  • Cb-Rhoの特定のcPrDは,アミロイド生成性を授与し,酵母PrDを機能的に置き換えることができる.
  • Cb-RhoはE. coliで溶解性,転写的に活性な形態と自己増殖性,機能的に損なわれたプリオン形態を採用した.
  • Cb-Rhoのプリオン形式は,ゲノム全体の重要なトランスクリプトーム変化を誘導した.

結論:

  • Cb-Rhoのような細菌のタンパク質は タンパク質ベースの遺伝要素として機能します
  • バクテリアのプリオンの発見は,その出現がユカリオットとバクテリアの進化の先にあることを示唆している.
  • この発見はプリオン生物学と その進化の歴史の 既知の範囲を広げています