ペリプラズマポリマーはビブリオ・コレラを曲げ,病原性を促進する

Thomas M Bartlett¹, Benjamin P Bratton², Amit Duvshani¹

¹Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.

Cell

|January 14, 2017

まとめ

研究者らは,細胞の曲線を司る Vibrio cholerae の骨格内部分である CrvA を発見しました. この発見は細菌がどのようにしてその形を作り運動性と病原性を高めるかを説明します

科学分野:

微生物学
細胞生物学
バクテリア病原性

背景:

病原性ビブリオコレラは人々の健康に重大な危険をもたらします
V. コレラの特徴的な曲った棒の形状とその基礎的なメカニズムは以前は知られていなかった.

研究の目的:

V. コレラ細胞の曲線の分子決定因子を特定し,特徴づけること.
細菌細胞の形状のメカニズムと機能的意義を明らかにする.

主な方法:

新しい曲率決定因子のCrvAの識別と特徴付け
ペプチドグライカンの動態を測定するために,QuASAR (サキュルス構造の再構築の定量分析) の開発.
crvAクオラム調節と細胞密度への影響の分析

主要な成果:

CrvAはペリプラズマフィラメントに自己組み立てられ,ペリスケルトの要素として作用します.
QuASARは,CrvAがペプチドグライカンの非対称な挿入を誘導し,細胞の曲線を引き起こすことを明らかにしました.
CrvAの発現はクオラム調節され,高細胞密度では曲線が増加する.

結論:

CrvAはV. choleraeの曲線を決定する最初の決定因子であり,骨盤の要素として機能する.
CrvAによる非対称なペプチドグリカン挿入は,曲線形成のメカニズムです.
CrvAは,ヒドロゲルのV.コレラ菌の運動性を高め,宿主コロニー化と病原化に寄与する.

キーワード:

細菌の細胞骨格細胞の形光Dアミノ酸病原性ペプチドグリカンペリスケルトン定量的な細胞生物学

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