ルース・サルコマウイルスカプシドタンパク質の管状組成の構造モデル
Jaekyun Jeon1, Xin Qiao1, Ivan Hung2
1Department of Physics, University of Central Florida , Orlando, Florida 32816, United States.
Journal of the American Chemical Society
|January 18, 2017
まとめ
Rous サルコマウイルス (RSV) のカプシドタンパク質 (CA) アセンブリに関する構造的な洞察は,ドメイン再構成がカプシドの曲線をどのように誘導するかを明らかにする. この研究は,RSV CAヘクサマーチューブの詳細な原子モデルを提供し,オルソレトロウイルスカプシド形成の理解に不可欠です.
科学分野:
- 構造生物学
- ウイルス学
- バイオ物理学
背景:
- オルソレトロウイルスカプシドタンパク質 (CA) はポリモルフなカプシドを形成し,その構造,組み立て,安定性は研究対象となっている.
- CAは,同型と異型の相互作用を媒介するN端末 (NTD) とC端末領域 (CTD) で構成され,カプシド表面にヘクサメリクタレットを形成します.
研究 の 目的:
- ヘクサマーチューブに組み込まれたロース・サーコマウイルス (RSV) のほぼ完全な固体NMR (ssNMR) 共振配分を決定する.
- RSV CAチューブルアームセットの原子解像度モデルを確立し,カプシドの曲線を駆動するメカニズムを解明する.
主な方法:
- 組み立てられたRSV CAヘクサマーチューブの共振配分のための固体NMR (ssNMR).
- 構造的な制約のために冷凍電子顕微鏡 (冷凍-EM).
- 原子解像度モデルを構築するための分子動力学フレキシブルフィッティング (MDFF) シミュレーション.
主要な成果:
- ssNMRの割り当ては,ループとCTD開始ヘリクスの有意な構成変化を,静的に乱れたドメイン間リンク器で明らかにした.
- MDFFモデルは,ssNMRと冷凍-EMデータと組み合わせて,カプシドの曲線が再構成されたNTD-CTDとCTDトリマーインターフェースから生じることを示しました.
- CTDのダイマーとトリマーのインターフェースの変動は,広範囲にわたるH結合とともに,CAヘクサマー格子の可塑性と変形に寄与する.
結論:
- RSVにおけるカプシド曲線は,主にNTDヘクサメリックタレットの周りのCTDの変位によって引き起こされる,ドメイン間インターフェースの再構成によって引き起こされる.
- HIVとRSVのCAアセンブリの螺旋接触角度の違いは,異なるカプシドアセンブリ経路を示唆する.
関連する概念動画
Protein Complex Assembly
17.0K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Many viruses self-assemble into a fully functional unit using the infected host cell to...
17.0K
Protein Complex Assembly
2.6K
2.6K
Coat Assembly and GTPases
4.6K
Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
4.6K
Assembly of Cytoskeletal Filaments
28.1K
Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...
28.1K
Rous Sarcoma Virus (RSV) and Cancer
6.5K
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
6.5K
Viral Structure
75.4K
Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
75.4K


