DNA オリガミに対する紫外線の影響
Haorong Chen1, Ruixin Li1, Shiming Li2
1School of Mechanical Engineering, Purdue University , West Lafayette, Indiana 47907, United States.
Journal of the American Chemical Society
|January 18, 2017
まとめ
紫外線はDNAの構造に影響します 適度な投与量では ストレスが軽減され 形状が回復しますが 高用量では 解体が起こります 波長が長いUVは 損傷のない写真再構成を可能にします
科学分野:
- ナノテクノロジー
- バイオ物理学
- 材料科学
背景:
- DNA オリガミはナノテクノロジーで 独自のナノスケールの形状を組み立てることができます
- 外部刺激に対する DNA オリガミの安定性と反応を理解することは,その応用にとって極めて重要です.
研究 の 目的:
- 紫外線 (UV) がDNAの形状に及ぼす影響を調査する.
- 構造的整合性と機能性に対する異なる紫外線波長と用量の影響を決定する.
主な方法:
- 様々な波長 (短波,中波,長波) のUV放射線にDNAオリガミの構造を曝露する.
- 構造変化の分析は,形状,欠陥,およびハイブリッド化能力を評価するテクニックを使用します.
- 光活性分子と結合したUVに対するDNAオリガミの反応の評価
主要な成果:
- 短波と中波の紫外線は DNA オリガミの光傷を誘発します
- 適度な紫外線は 内部のストレスを軽減し 設計された形状を復元し ハイブリダイゼーションを危うくしません
- 高濃度の紫外線は ステーパルの解離と構造的な崩壊を引き起こします
- 波長が長いUVは DNAの形状に 影響しません
結論:
- 紫外線はDNAの オリガミ構造を 操作するために利用できます
- 適度な紫外線は ストレス緩和メカニズムとして機能し 構造の忠誠性を高めます
- 高い紫外線線は有害で 構造的な崩壊を引き起こします
- 長い波長のUVは,構造的整合性を保ち,光活性分子と組み合わせたときに制御された写真再構成の経路を提供します.
関連する概念動画
Mutations
45.0K
Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
45.0K
Nucleotide Excision Repair
41.4K
Overview
41.4K
Single-Strand DNA Binding Proteins
17.0K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
17.0K
DNA Topoisomerases
36.8K
Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
36.8K
DNA as a Genetic Template
28.3K
Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
28.3K
DNA Helicases
24.7K
DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
24.7K


