オートホーナル化学結合のための高信頼性複製能力のDNA背骨の分子要求

Arun Shivalingam¹, Agnes E S Tyburn¹, Afaf H El-Sagheer^1,2

¹Department of Chemistry, Chemistry Research Laboratory, University of Oxford , 12 Mansfield Road, Oxford OX1 3TA, U.K.

Journal of the American Chemical Society

|January 19, 2017

まとめ

研究者たちは遺伝子の複製を理解するために人工DNAの脊椎を調べましたアミド骨格の形成は DNA合成に有望な結果を示し複製の信頼性にはリン酸群が欠かせないことを明らかにしました

科学分野:

分子生物学
合成化学
バイオテクノロジー

背景:

遺伝情報の複製におけるフォスフォディエスターの進化的優位性は不明である.
バイオコンパティブルな改変DNAを組み立てるには,新しい化学結合戦略の開発が不可欠です.

研究の目的:

複製に影響を与える人工DNAの分子特性を調査する.
改造されたDNA合成のための新しい化学結合方法を開発する.

主な方法:

多様な人工結合を持つオリゴヌクレオチドの合成
プリマーの拡張,PCR,および深層配列の使用による複製運動と忠誠性の評価.
化学的結合戦略は,アミド骨格形成と銅 (I) 触媒アルキンアジドサイクル添加 (CuAAC) "クリック"結合を含む.

主要な成果:

CuAAC クリック・リガーションに直角な,アミド・バックボーン形成に基づく新しいリガーション法が開発された.
人工結合による複製の確率は,骨幹の柔軟性,ステリック因子,ポリメラーゼの水素結合に依存する.
特定のトリアゾールとアミドDNAの背骨は効率的な複製を示し,リン酸群は不可欠ではないことを示唆している.

結論:

フォスファート群はDNA複製に欠かせないので既存のパラダイムに異議を唱えます
アミド骨格形成は合成DNAの組み立てに新しい戦略を提示します
発見は合成生物学と新型核酸ベースのシステムの設計に影響を与えます

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