化学プロファイルに基づいて細胞を分類する:単細胞質量スペクトロメトリーの進歩
Troy J Comi1, Thanh D Do1, Stanislav S Rubakhin1
1Department of Chemistry and the Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign , Urbana, Illinois 61801, United States.
Journal of the American Chemical Society
|January 31, 2017
まとめ
質量スペクトロメトリーは単細胞の詳細な分析を可能にし,健康と病気を理解するために重要な細胞の異質性を明らかにします. 将来の技術は,大きな細胞集団を分析する上で,より高いスループットを約束します.
科学分野:
- 生物化学
- 細胞生物学
- 分析化学
背景:
- 細胞の異質性は,ホメオスタシスと疾患の発達に関する洞察を提供します.
- 単細胞を分析する際の課題は,少量のサンプルと低量の分析物です.
- 既存の増幅方法は,単細胞でのメタボライトとタンパク質の包括的な特徴付けには不十分である.
研究 の 目的:
- マススペクトロメトリーベースの単細胞分析の現在の傾向と進歩をレビューする.
- 次世代の単細胞測定を可能にする技術を特定する.
- 細胞の異質性を理解する上で質量スペクトロメトリーの重要性を強調する.
主な方法:
- 細胞代謝物の敏感で高ダイナミック範囲の分析のための質量スペクトロメトリー (MS).
- MSベースの単細胞分析における現在の機器と技術的進歩のレビュー
- 単細胞のサンプル採取を容易にする技術に重点を置く.
主要な成果:
- 質量スペクトロメトリは個々の細胞代謝物のプロファイリングの鍵となる技術である.
- MS機器の進歩により,単細胞分析の感度とスループットが向上しています.
- 新しい技術の開発は,高通量単細胞の定期的な測定に不可欠です.
結論:
- 質量スペクトロメトリーは細胞の異質性を解剖するのに不可欠です.
- 単細胞分析の現在の限界を克服するために,技術革新を継続する必要があります.
- 単細胞レベルでの生物学的システムの理解を深めるため
関連する概念動画
Overview Of Cell Separation And Isolation
7.9K
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
7.9K
Flow Cytometry
16.8K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
In...
16.8K
Tandem Mass Spectrometry
2.8K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.8K
MALDI-TOF Mass Spectrometry
7.3K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
7.3K
Mass Spectrometry: Complex Analysis
2.0K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
2.0K


