空気中の高レベルの触媒活性を維持する表面固定化酵素
Somayesadat Badieyan1, Qiuming Wang1, Xingquan Zou1
1Department of Chemistry, University of Michigan , Ann Arbor, Michigan 48109, United States.
Journal of the American Chemical Society
|February 14, 2017
まとめ
空気中の酵素活動は,ハロアルカン脱ハロゲネーゼを新しい表面に固定することによって達成される. この工学的に作られた材料は タンパク質の構造を保ち "水なし"の酵素の活性化を促進します
科学分野:
- 生物化学
- 材料科学
- 化学工学
背景:
- 酵素は通常,活動のために水性バッファを必要とし,水性でない環境での使用を制限します.
- 空気や環境の湿度で機能する酵素の開発は,様々な産業やバイオテクノロジーの応用に不可欠です.
- 水のない酵素システムは,新しい触媒プロセスと安定性の改善の可能性を提供します.
研究 の 目的:
- 空気中の触媒活動をサポートする精密な酵素不動化のための表面を設計する.
- 水性バッファがない場合に酵素機能を強化する新しいポリマーサポーターの可能性を調査する.
- エンジニアリングされた表面が酵素の構造と活性を維持するメカニズムを探求する.
主な方法:
- ハロアルカンデハロゲネーゼの化学的に精密な共性不動化は,ポリ ((ソルビトールメタクリlate) 鎖を表示する表面に.
- 周囲の湿度で空気中の固定酵素による1‐ブロモプロパンのガス相脱酸化の測定.
- 溶解酵素粉と水性バッファーの酵素との活性比較
主要な成果:
- 解凍酵素粉と比較して,固定されたハロアルカン脱ハロゲネーゼの活性が約40倍増加した.
- 空気中の酵素の活性度は,水性バッファーの活性量の約25%に達した.
- タンパク質と水の相互作用を模倣して酵素を安定させる.
結論:
- エンジニアリングされた表面での化学的に正確な固定化は,空気中の酵素活動を可能にします.
- ポリソルビトールメタクリlate) は,安定した活性化剤を作るための有望な材料です.
- 水がない
- 酵素システムです
- このアプローチは,様々な環境における酵素の潜在的応用を大幅に拡大します.
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