シアノバクチン RiPP 自然製品における酵素によるNおよびC保護
Debosmita Sardar1, Yue Hao2, Zhenjian Lin1
1Department of Medicinal Chemistry, University of Utah , Salt Lake City, Utah 84112, United States.
Journal of the American Chemical Society
|February 15, 2017
まとめ
研究者はペプチドを保護するために新しい酵素であるAgeMTPTを用いてエアルギノサミドBを合成した. この研究は,シアノバクチン生物合成とマクロサイクリングメカニズムの理解を進める.
科学分野:
- 生物化学
- 合成生物学
- 自然製品の生物合成
背景:
- サイアノバクチンは,N端とC端のマクロサイクリングによって保護された循環ペプチドである.
- ペプチドの改変メカニズムを理解することは,合成生物学の応用にとって極めて重要です.
研究 の 目的:
- 自然産物であるアルーギノサミドBを合成する.
- シアノバクチンのマクロサイクリングの酵素機構を調査する.
- 新しい2機能酵素AgeMTPTの有用性を調べるため
主な方法:
- NとC末端のペプチドの保護のために新しい二機能酵素AgeMTPTを使用した.
- 総合成のためにモジュラー生物合成酵素を用いる.
- ペプチド改変とマクロサイクリングの酵素的メカニズムを調査した.
主要な成果:
- エアルーギノサミドBの全合成を達成した.
- 線形シアノバクチンの生体合成が実証されている.
- トランスペプチデーションのための水排除に対する運動的可変性仮説を支持する証拠を提供した.
結論:
- 新しい酵素AgeMTPTは,生物合成のための線形ペプチドの保護を可能にします.
- この研究は,シアノバクチンのマクロサイクリングメカニズムの重要な側面を明らかにしています.
- 発見はペプチド天然産物合成と合成生物学というより広い分野に寄与する.
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