関連する実験動画
Updated: Mar 7, 2026
09:37
An Integrated Approach for Microprotein Identification and Sequence Analysis
Published on: July 12, 2022
4.0K
正確な5'端を持つ人間の長い非コーディングRNAのアトラス
Chung-Chau Hon1, Jordan A Ramilowski1,2, Jayson Harshbarger1,2
1RIKEN Center for Life Science Technologies (Division of Genomic Technologies), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045 Japan.
Nature
|February 28, 2017
まとめ
この研究は,ヒトの長い非コーディングRNA (lncRNA) アトラスを包括的に提示し,数千の潜在的に機能するlncRNAとその遺伝子調節と疾患における役割を特定しています.
科学分野:
- ゲノミクス
- トランスクリプトミクス
- エピジェノミクス
背景:
- 長い非コーディングRNA (lncRNAs) は,主に未知の機能を持つ多様な分子クラスです.
- 既存のトランスクリプトコレクションは断片化されており,lncRNAレパートリーの包括的な理解を妨げています.
研究 の 目的:
- ヒトの lncRNA 遺伝子のアトラスを作成する
- ゲノムとエピジェノームの特徴に基づいて lncRNA を分類する.
- 遺伝子調節と疾患における lncRNA の機能的役割を調査する.
主な方法:
- FANTOM5の遺伝子発現 (CAGE) データと複数のトランスクリプトの収集を統合する.
- lncRNAのゲノムと表遺伝子分類
- 様々なヒトの細胞タイプと組織における lncRNA発現プロフィールの分析.
- 遺伝子,表現,保存データの統合
主要な成果:
- 人間のIncRNA遺伝子27,919の高信頼性アトラスを生成した.
- ほとんどのインタージェニック lncRNAは強化剤から発現することが判明した.
- lncRNAが特徴に関連したSNPを重複すると,特徴に関連する細胞タイプ特有の発現が示された.
- eQTLに関連したSNPが重複するlncRNAは,標的mRNAと共発され,規制的な役割を示唆した.
- 19, 175 の潜在的に機能する lncRNA が特定されました.
結論:
- この研究は lncRNA 研究に貴重なリソースを提供します.
- lncRNAは,転写調節において重要な役割を果たし,様々な疾患に関与しています.
- このアトラスは,lncRNAのさらなる機能的特徴付けを容易にする.
関連する概念動画
02:39lncRNA - Long Non-coding RNAs
10.0K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
10.0K
02:39lncRNA - Long Non-coding RNAs
3.8K
3.8K
02:35RACE - Rapid Amplification of cDNA Ends
7.4K
Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
7.4K
03:18Non-LTR Retrotransposons
13.6K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
13.6K
03:21RNA-seq
12.3K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.3K
02:24Ribosome Profiling
4.2K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.2K