ホロ酵素形成に必要な媒介体の構造と再配置
Kuang-Lei Tsai1, Xiaodi Yu1, Sneha Gopalan2
1Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla California, USA.
Nature
|February 28, 2017
まとめ
メディエーター・コンプレックス
科学分野:
- 分子生物学
- 構造生物学
- 生物化学
背景:
- メディエーター複合体は,真核生物間のRNAポリメラーゼII転写を調節する重要な共同活性化剤である.
- メディエーターの構造と相互作用を理解することは,転写開始と信号伝達におけるその役割を解読する鍵です.
研究 の 目的:
- Schizosaccharomyces pombe Mediator複合体の高解像度構造を解明する
- RNAポリメラーゼIIホロ酵素形成中のメディエーターの構造的再配置を調査する.
主な方法:
- クリオ電子顕微鏡 (cryo-EM) を使用して,Mediatorの構造を4. 4 Åの解像度で決定した.
- 比較構造分析は,メディエーターRNAポリメラーゼIIホロ酵素マップ (解像度7. 8 Å) で行われた.
主要な成果:
- 保存された個々のメディエーターサブユニットは4. 4 Åの冷凍-EMマップ内で解消された.
- Med14サブユニットはメディエーターモジュール (ヘッド,ミドル,テール) を接続する中央の支架として機能します.
- ホロ酵素形成中にメド14の構造変化が観察され,メディエーターの再配置が容易になりました.
結論:
- この研究は,メディエーター構造を組織し,ホロ酵素の組み立てを促進するメディ14の重要な役割を明らかにしています.
- メディエーターの構造的柔軟性とモジュール間の通信は,トランスクリプションに規制信号を統合するために不可欠です.
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