プロファージWO遺伝子は,ウォルバキア誘発の細胞質不適合性を再現し,強化する
Daniel P LePage1, Jason A Metcalf1, Sarah R Bordenstein1
1Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee 37235, USA.
Nature
|February 28, 2017
まとめ
研究者らは,ウォルバキア誘発の細胞質不適合性に関与する2つの遺伝子, cifAとcifBを特定した. この発見は 繁殖の操作と ヴォルバキアの理解を前進させました
科学分野:
- 微生物学
- 遺伝学
- 進化生物学
背景:
- ヴォルバキアは 細胞内細菌で 脊椎動物に感染し 繁殖を操作して 流行を増加させます
- 細胞プラズマ不適合性 (CI) は,特定の交配において胚の致死性を引き起こす,Wolbachiaによって引き起こされる一般的な生殖操作である.
- CIの遺伝的根拠は,共生生物による種化と害虫制御におけるその重要性にもかかわらず,未だに曖昧である.
研究 の 目的:
- ウォルバキア媒介による細胞プラズマ不適合性の 細菌の遺伝子を特定する.
- トランスジェニックのアプローチを使用して,識別された遺伝子を機能的に検証する.
主な方法:
- プロファージWO遺伝子の比較ゲノミクス
- ドロソフィラ・メラノガスターにおける候補遺伝子 (cifAとcifB) のトランス遺伝子発現
- トランスジェニックなハエの交配における胚の死亡率の評価
主要な成果:
- プロファージWOの真核結合モジュールから2つの共分散遺伝子, cifAと cifBが特定されました.
- 感染していない雄のドロソフィラにCIFAとCIFBの二重発現が再現され,CIが強化された.
- 観察された胚死亡率は,野生型のCIを模倣し,Wolbachiaに感染した胚によって救出されました.
結論:
- 遺伝子 cifAとcifBは,Wolbachiaにおける細胞質不適合性の主要な要因である.
- この発見は ヴォルバキアの生殖操作に関する 遺伝子研究の新たな道を開きます
- デング熱やジカウイルスを 伝達するアーボウイルスベクトルの制御に ウォルバキアの利用を 支持しています
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