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関連する概念動画

Notch Signaling Pathway03:14

Notch Signaling Pathway

6.8K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
6.8K
Ligand Binding Sites02:40

Ligand Binding Sites

15.5K
Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
15.5K
Ligand Binding Sites02:40

Ligand Binding Sites

9.0K
9.0K
Crystal Field Theory - Octahedral Complexes02:58

Crystal Field Theory - Octahedral Complexes

31.4K
Crystal Field Theory
To explain the observed behavior of transition metal complexes (such as colors), a model involving electrostatic interactions between the electrons from the ligands and the electrons in the unhybridized d orbitals of the central metal atom has been developed. This electrostatic model is crystal field theory (CFT). It helps to understand, interpret, and predict the colors, magnetic behavior, and some structures of coordination compounds of transition metals.
CFT focuses on...
31.4K
Valence Bond Theory02:42

Valence Bond Theory

11.4K
Coordination compounds and complexes exhibit different colors, geometries, and magnetic behavior, depending on the metal atom/ion and ligands from which they are composed. In an attempt to explain the bonding and structure of coordination complexes, Linus Pauling proposed the valence bond theory, or VBT, using the concepts of hybridization and the overlapping of the atomic orbitals. According to VBT, the central metal atom or ion (Lewis acid) hybridizes to provide empty orbitals of suitable...
11.4K
Conserved Binding Sites01:49

Conserved Binding Sites

5.2K
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
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関連する実験動画

Updated: Mar 6, 2026

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
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Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

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Notch-Jagged複合構造は,リガンドのセンシビリティの調節にキャッチボンドを伴う

Vincent C Luca1,2, Byoung Choul Kim3,4, Chenghao Ge5

  • 1Departments of Molecular and Cellular Physiology and Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.

Science (New York, N.Y.)
|March 4, 2017
PubMed
まとめ

ノッチ受容体の活性化には,機械的な力とグリコシル化が不可欠である. 新しい構造データは,Notch1に結合するJagged1が,細胞の運命を決定するためにこれらの力をどのように使用しているかを明らかにしています.

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Author Spotlight: Unveiling the Structural and Dynamic Aspects of Glycan Molecular Recognition
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A Protocol for Computer-Based Protein Structure and Function Prediction
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A Protocol for Computer-Based Protein Structure and Function Prediction

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関連する実験動画

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Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
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Published on: January 2, 2018

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A Protocol for Computer-Based Protein Structure and Function Prediction
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A Protocol for Computer-Based Protein Structure and Function Prediction

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科学分野:

  • 細胞生物学
  • 構造生物学
  • 生物化学

背景:

  • 細胞の運命を決定する上で重要なものです.
  • この経路は機械的力とタンパク質のグリコシル化に依存する点でユニークです.
  • 分子相互作用を理解することは ノッチ経路の規則を解読する鍵です

研究 の 目的:

  • Notch1とJagged1 (Jag1) の相互作用の構造的基礎を明らかにする.
  • Notch1-Jag1結合における機械的力とグリコシレーションの役割を調査する.
  • Notch1のJag1への結合とデルタ型4 (DLL4) リガンドを比較する.

主な方法:

  • 2.5アングストロムの解像度でX線結晶撮影
  • タンパク質とタンパク質の結合界面の分析
  • タンパク質の糖化変化の特徴 (O結合フコゼ)

主要な成果:

  • Notch1-Jag1 細胞外複合体の詳細な構造が決定された.
  • Notch1 EGFドメイン8と12のO結合フコースは,Jag1 EGF3とC2ドメインと相互作用する.
  • Jag1は,機械的な力によって影響を受け,Notch1の結合時にキャッチボンドの振る舞いを示します.
  • Notch1は,DLL4結合と比較して,Jag1結合に異なるドメインを使用しています.

結論:

  • Notch1- Jag1の相互作用には,機械的な力や特定のグリコシル化パターンが重要です.
  • Jag1のキャッチ・ボンドの振る舞いは,ノッチ・シグナリングの力依存の調節を可能にします.
  • これは,リガンドの差別化と,メカニカルシグナルを通してノッチシグナルの強化のためのメカニズムを提供します.