ゴルギ複合体の最も横断的な水槽:分泌およびプラズマ膜タンパク質の分類のための区画
L Orci1, M Ravazzola, M Amherdt
1Institute of Histology and Embrology, University of Geneva, Switzerland.
Cell
|December 24, 1987
まとめ
研究者は,免疫電子顕微鏡を用いて,インスリンおよびヘマグルチニンタンパク質の経路を追跡した. 彼らは,タンパク質の分類がゴルジ装置のトランスモストシスターナで起き,調節された経路と構成経路を分離することを発見しました.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- タンパク質の密輸 タンパク質の密輸
背景:
- タンパク質を調節された経路または構成経路に細胞内分類するメカニズムは,ほとんど解明されていないままです.
- これらの経路を理解することは,細胞機能と疾患の研究に不可欠です.
研究 の 目的:
- タンパク質が調節された分泌経路 (例えばインスリン) と構成的分泌経路 (例えばヘマグルチニン) に分類される細胞内部位を決定する.
- これらの2つの異なるタンパク質の細胞内の輸送経路を視覚化し,区別する.
主な方法:
- 免疫電子顕微鏡を用いて,インスリンとヘマグルーチニンの位置と動きを追跡した.
- 細胞を分析して,ゴルギのスタックおよびその後の輸送ベジクル内の両方のタンパク質の分布を観察しました.
主要な成果:
- インスリンとヘマグルチニンは,ゴルギの水槽の中にランダムに分布していることが判明しました.
- ゴルギの外側では,インスリンが密集した核の分泌粒に局所化し,ヘマグルーチニンは透明な膀に発見されました.
- ヘマグルチニンを運ぶ透明な膀は,内細胞のトレーサーで有意なラベルを表示せず,外細胞のキャリアであることを示唆しました.
結論:
- タンパク質が調節された経路と構成経路に分類されるのは,ゴルジ装置のクラトリンで覆われたトランスモストシスタナで起こります.
- この分岐点では,プロインスリン包装が開始され,構成分泌を目的としたヘマグルチニンなどのタンパク質から分離されます.
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