組織的なヒトILC前駆体は,組織ILCの差別化のための基板を提供します.
Ai Ing Lim1, Yan Li2, Silvia Lopez-Lastra3
1Innate Immunity Unit, Institut Pasteur, 75724 Paris, France; Inserm U1223, 75015 Paris, France; Université Paris-Diderot, Sorbonne Paris Cité, 75205 Paris, France.
Cell
|March 12, 2017
まとめ
科学者はヒトの血液循環と組織内生リンパ性細胞前駆体 (ILCP) を発見した. これらの前駆体は,すべての成熟した先天性リンパ性細胞 (ILC) のサブセットを in situ で生成し,ILC 発達の新しいモデルをサポートします.
科学分野:
- 免疫学
- 細胞生物学
背景:
- 生まれながらのリンパ性細胞 (ILC) は,多様な機能を持つ重要な免疫細胞です.
- ILC がその前駆体 (ILCP) から成熟した組織内細胞へと進化する経路は完全に理解されていません.
研究 の 目的:
- 人間の先天性リンパ球前駆体 (ILCP) を特定し,特徴づけること.
- ヒトのILCPを様々なILCサブセットに区分する可能性を調査する.
- "ILCポエシス"と呼ばれる組織におけるILC発達の過程を明らかにする.
主な方法:
- 人間における循環および組織ILCPの識別と特徴付け.
- ILCPの可能性を評価するために,in vitroおよびin vivoの差別化アッセイ.
- ILCPの転写因子発現と表遺伝子状態の分析
主要な成果:
- ヒトのILCPは,シグネチャー遺伝子の位置で平衡した表遺伝子構成で特定されました.
- ILCPは,すべての主要なILCサブセット (NK,ILC1,ILC2,ILC3) の強力な生成を in vitroおよびin vivoで実証した.
- ILCPは,RORCのような重要な転写因子がない場合でも,分化の可能性を保持した.
結論:
- 組織内の様々なILCサブセットに系統的なILCPが分化する"ILC-ポエシス"のモデルが提案されています.
- 局所的な環境信号は,組織特有のニーズに対応してILCPの差異化を誘導する可能性がある.
- この発見は,先天的な免疫細胞の発達と組織適応の理解における重大なギャップを明らかにしています.
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