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関連する概念動画

Membrane Transporters01:31

Membrane Transporters

18.6K
Transporters are essential membrane transport proteins with functions related to cell nutrition, homeostasis, communication, etc. Approximately 7% of all genes in the human genome code for transporters or transporter-related proteins.
Transporters are mainly composed of alpha-helices, built from bundles of ten or more helices traversing the plasma membrane. The solute-binding sites are located midway, where some of the helices are broken or distorted, making space for the binding site through...
18.6K
Facilitated Diffusion01:16

Facilitated Diffusion

1.5K
The plasma membrane, a critical structure in cellular biology, houses an array of transporters, or carrier proteins, interspersed within its lipid bilayer. These proteins play a crucial role in solute transport through facilitated diffusion, a form of passive diffusion that uses transporters to move the molecules across the membrane.
In this process, substrates such as organic compounds and ions interact with a transporter on one side, triggering conformational changes in proteins that enable...
1.5K
ABC Transporters: Importer01:27

ABC Transporters: Importer

3.6K
ATP-binding cassette or ABC transporters are a class of ATP-driven pumps that hydrolyze ATP to move solutes across the membrane. They can be grouped into importers and exporters. While exporters are present in all domains of life, importers exist only in bacteria and some plants.
In bacteria, based on the number of transmembrane helices and the chemical nature of their substrates, the ABC importers can be divided into three types:
3.6K
Carrier-Mediated Transport01:06

Carrier-Mediated Transport

1.4K
Carrier-mediated transport is a pivotal process in drug absorption, particularly for lipid-insoluble drugs, and encompasses facilitated diffusion and active transport. Facilitated diffusion allows drugs to move along their concentration gradient without energy expenditure, while active transport utilizes ATP to drive drug movement against this gradient.
Active transport involves two types of membrane-spanning transporters: uptake and efflux. Uptake transporters are expressed in the small...
1.4K
Active Transport01:14

Active Transport

2.5K
Active transport is a critical biological process that allows cells to move solutes against an electrochemical gradient. This process requires direct energy input and is characterized by its selectivity, saturability, and susceptibility to competitive inhibition.
Primary active transporters, like Na+, K+ and -ATPase, directly utilize ATP to move ions across the membrane. These transporters play significant roles in various physiological processes. For instance, Na+, K+ and -ATPase maintain...
2.5K
ABC Transporters: Exporter01:31

ABC Transporters: Exporter

6.9K
ATP-binding cassette or ABC transporter is the largest superfamily of integral membrane proteins. The transporters have transmembrane-binding domains (TMDs) and nucleotide-binding domains (NBDs). The TMDs are specific to their substrates, whereas the NBDs are similar to engines that complete ATP hydrolysis to complete the substrate transport. They can be full transporters consisting of two TMDs and NBDs, half transporters with one TMD and NBD, while some encoded with a single TMD or NBD are...
6.9K

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関連する実験動画

Updated: Mar 6, 2026

Author Spotlight: Expression and Purification of Human Solute Carrier Transporters Using Codon-Optimized Genes
07:10

Author Spotlight: Expression and Purification of Human Solute Carrier Transporters Using Codon-Optimized Genes

Published on: September 29, 2023

4.5K

運搬 者 たち が 明らか に なっ た

Jyh-Yeuan Lee1, Daniel M Rosenbaum2

  • 1Eugene McDermott Center for Human Growth and Development, The University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.

Cell
|March 12, 2017
PubMed
まとめ
この要約は機械生成です。

ATP結合カセットサブファミリーC (ABCC) トランスポーターに関する構造的洞察は,薬物耐性およびイオン輸送におけるその多様な機能の背後にあるメカニズムを明らかにする. クリオ-エム構造は 輸送器の動作を 解明します

さらに関連する動画

Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles
13:16

Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles

Published on: December 31, 2019

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Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers
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Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers

Published on: October 17, 2013

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関連する実験動画

Last Updated: Mar 6, 2026

Author Spotlight: Expression and Purification of Human Solute Carrier Transporters Using Codon-Optimized Genes
07:10

Author Spotlight: Expression and Purification of Human Solute Carrier Transporters Using Codon-Optimized Genes

Published on: September 29, 2023

4.5K
Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles
13:16

Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles

Published on: December 31, 2019

9.9K
Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers
18:57

Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers

Published on: October 17, 2013

47.5K

科学分野:

  • 生物化学
  • 構造生物学
  • 分子生物学

背景:

  • ATP結合カセット (ABC) トランスポーターは,様々な細胞プロセスに関与する重要な膜タンパク質です.
  • ABCサブファミリーC (ABCC) は,多剤耐性およびイオンチャネル調節に関与しています.
  • ABCCのトランスポーター構造を理解することは,その多様な生理学的役割を解読する鍵です.

研究 の 目的:

  • ABCC トランスポーターの機能的多様性の構造的基礎を明らかにする.
  • 主要なABCCトランスポーターの原子または近原子解像度構造を提供する.
  • ABCCトランスポーターの既知の機能と構造特性を相関させる.

主な方法:

  • 単粒子の冷凍電子顕微鏡 (cryo-EM) が使用された.
  • 3つの重要なABCCトランスポーター:MRP1,SUR1,CFTRの高解像度構造が決定されました.
  • 構造データは,重要な機能領域と構成状態を特定するために分析されました.

主要な成果:

  • MRP1,SUR1,CFTRの原子または近原子解像度構造が得られました.
  • これらのABCCトランスポーターの多様な機能を説明する詳細な構造的特徴が明らかにされました.
  • この発見は,ABCCのトランスポーターメカニズムを理解するための構造的枠組みを提供する.

結論:

  • クリオ-エム構造は,ABCCのトランスポーター機能に 前例のない洞察を提供します.
  • 構造的多様性は,ABCCトランスポーターの多様な生理学的役割と直接相関しています.
  • これらの発見は,多剤耐性およびイオン輸送メカニズムの理解を進める.